NDLI: Characterization of a thermostable and alkaline xylanase from Bacillus sp. and its bleaching impact on wheat straw pulp

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Characterization of a thermostable and alkaline xylanase from Bacillus sp. and its bleaching impact on wheat straw pulp

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Characterization of a thermostable and alkaline xylanase from Bacillus sp. and its bleaching impact on wheat straw pulp

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Saleem, Mahjabeen Aslam, Farheen Akhtar, Muhammad Saleem Tariq, Mohsin Rajoka, Muhammad Ibrahim
Description	Country affiliation: Pakistan Author Affiliation: Saleem M ( Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, 54590, Pakistan. mahjabeensaleem1@hotmail.com)
Abstract	Delignification efficacy of xylanases to facilitate the consequent chemical bleaching of Kraft pulps has been studied widely. In this work, an alkaline and thermally stable cellulase-less xylanase, derived from a xylanolytic Bacillus subtilis, has been purified by a combination of gel filtration and Q-Sepharose chromatography to its homogeneity. Molecular weight of the purified xylanase was 61 kDa by SDS–PAGE. The purified enzyme revealed an optimum assay temperature and pH of 60°C and 8.0, respectively. Xylanase was active in the pH range of 6.0–9.0 and stable up to 70°C. Divalent ions like $Ca^{2+},$ $Mg^{2+}$ and $Zn^{2+}$ enhanced xylanase activity, whereas $Hg^{2+},$ $Fe^{2+},$ and $Cu^{2+}$ were inhibitory to xylanase at 2 mM concentration. It showed $K_{m}$ and $V_{max}$ values of 9.5 mg/ml and 53.6 μmol/ml/min, respectively, using birchwood xylan as a substrate. Xylanase exhibited higher values of turn over number $(K_{cat})$ and catalytic efficiency $(K_{cat}/K_{m})$ with birchwood xylan than oat spelt xylan. Bleach-boosting enzyme activity at 30 U/g dry pulp displayed the optimum bio-delignification of Kraft pulp resulting in 26.5% reduction in kappa number and 18.5% ISO induction in brightness at 55°C after 3 h treatment. The same treatment improved the pulp properties including tensile strength and burst index, demonstrating its potential application in pre-bleaching of Kraft pulp.
File Format	HTM / HTML
ISSN	09593993
Issue Number	2
Volume Number	28
e-ISSN	15730972
Journal	World Journal of Microbiology and Biotechnology
Language	English
Publisher	Springer
Publisher Date	2012-02-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Microbiology Temperature Enzyme Stability Metabolism Journal Article Discipline Biotechnology Bacterial Proteins Triticum Chemistry Bacillus Xylosidases Hydrogen-ion Concentration Enzymology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

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