NDLI: Keratinases and sulfide from Bacillus subtilis SLC to recycle feather waste.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Cedrola, Sabrina Martins Lage de Melo, Ana Cristina Nogueira Mazotto, Ana Maria Lins, Ulysses Zingali, Russolina Benedeta Rosado, Alexandre Soares Peixoto, Raquel S. Vermelho, Alane Beatriz
Spatial Coverage	Brazil
Description	Country affiliation: Brazil Author Affiliation: Cedrola SM ( Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes (IMPG), Bloco I, Centro de Ciências da Saúde (CCS), Universidade Federal do Rio de Janeiro (UFRJ), Cidade Universitária, Ilha do Fundão, Rio de Janeiro, RJ 21941-590, Brazil.)
Abstract	The aim of this study is to investigate the culture conditions of chicken feather degradation and keratinolytic enzyme production by the recently isolated Bacillus subtilis SLC and to evaluate the potential of the SLC strain to recycle feather waste discarded by the poultry industry. The SLC strain was isolated from the agroindustrial waste of a poultry farm in Brazil and was confirmed to belong to Bacillus subtilis by rDNA gene analysis. There was high keratinase production when the medium was at pH 8 (280 U ml(-1)). Activity was higher using the inoculum propagated for 72 h on 1% whole feathers supplemented with 0.1% yeast extract. In the enzymatic extract, the keratinases were active in the pH range from 2.0 to 12.0 with a maximum activity at pH 10.0 and temperature 60°C. For gelatinase the best pH was 5.0 and the best temperature was 37°C. All keratinases are serine peptidases. The crude enzymatic extract degraded keratin, gelatin, casein, and hemoglobin. Scanning electron microscopy showed Bacillus cells adhered onto feather surfaces after 98 h of culture and degraded feather filaments were observed. MALDI-TOF mass spectrometric analysis showed multiple peaks from 522 to 892 m/z indicating feather degradation. The presence of sulfide was detected on extracellular medium probably participating in the breakdown of sulfide bridges of the feather keratin. External addition of sulfide increased feather degradation.
File Format	HTM / HTML
ISSN	09593993
Issue Number	3
Volume Number	28
e-ISSN	15730972
Journal	World Journal of Microbiology and Biotechnology
Language	English
Publisher	Springer
Publisher Date	2012-03-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Research Support, Non-u.s. Gov't Temperature Microbiology Rna, Ribosomal, 16s Molecular Sequence Data Bacillus Subtilis Journal Article Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization Feathers Sulfides Dna, Ribosomal Dna, Bacterial Classification Brazil Genetics Hydrogen-ion Concentration Discipline Microbiology Microscopy, Electron, Scanning Isolation & Purification Enzyme Stability Metabolism Sequence Analysis, Dna Discipline Biotechnology Bacterial Adhesion Chemistry Animals Chickens Medical Waste Disposal Peptide Hydrolases Enzymology Methods
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

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Keratinases and sulfide from Bacillus subtilis SLC to recycle feather waste

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