NDLI: Activation of PDGFR and EGFR promotes the acquisition of a stem cell-like phenotype in schwannomas.

Please wait, while we are loading the content...

Year: 2017 Volume: 38

Year: 2016 Volume: 37

Year: 2015 Volume: 36

Year: 2014 Volume: 35

Year: 2013 Volume: 34

Year: 2012 Volume: 33

Year: 2012 Volume: 33 Issue: 9

Imaging case of the month: Cystic lesions of the petrous apex: identification based on magnetic resonance imaging characteristics.

Imaging case of the month: Bilateral internal auditory canal melanoma.

Temporal bone histopathology case of the month: Acute unilateral facial paralysis in child from intra-aural hard tick.

Imaging case of the month: Abnormalities of the cochlear nerves and internal auditory canals in pontine tegmental cap dysplasia.

Temporal bone histopathology case of the month: Malignant peripheral nerve sheath tumor arising within vestibular schwannoma.

Activation of PDGFR and EGFR promotes the acquisition of a stem cell-like phenotype in schwannomas.

The round window: is it the 'cochleostomy' of choice? Experience in 130 consecutive cochlear implants.

Stimulation rate reduction and auditory development in poorly performing cochlear implant users with auditory neuropathy.

Petrous bone fractures violating otic capsule.

Influence of an intraoperative perilymph gusher on cochlear implant performance in children with labyrinthine malformations.

Mannitol protects hair cells against tumor necrosis factor -induced loss.

Otopathology in Osteogenesis Imperfecta.

Sex and age associations with vestibular schwannoma size and presenting symptoms.

Evaluation of masking effects on speech perception in patients with unilateral chronic tinnitus using the hearing in noise test.

Microsurgery versus stereotactic radiation for small vestibular schwannomas: a meta-analysis of patients with more than 5 years' follow-up.

Expression of anion exchangers in cultured human endolymphatic sac epithelia.

Effects of hyperbaric oxygen and dexamethasone on proinflammatory cytokines of rat cochlea in noise-induced hearing loss.

Experimental video analysis of eye blink reflex in a primate model.

Hearing preservation in management of epidermoids of the cerebellopontine angle: CPA epidermoids and hearing preservation.

How to uncover the covert saccade during the head impulse test.

Balloon catheter dilatation of Eustachian tube: a preliminary study.

Oral steroid treatment for hearing improvement in Ménière's disease and endolymphatic hydrops.

Intraoperative round window recordings to acoustic stimuli from cochlear implant patients.

High-fidelity, inexpensive surgical middle ear simulator.

Concerto Pin: a novel concept of cochlear implant fixation.

Comparison of regeneration of the chorda tympani nerve and gustatory function recovery after severing the nerve between pediatric and adult patients.

Imaging case of the month: Metastatic prostate adenocarcinoma presenting as hearing loss and disequilibrium.

Association between hearing loss and saccular dysfunction in older individuals.

Risk of developing sudden sensorineural hearing loss in diabetic patients: a population-based cohort study.

Minimal access cochlear implant fixation: temporalis pocket with a plate.

Presbycusis occurs after cochlear implantation also: a retrospective study of pure tone thresholds over time.

Otolithic membrane damage in patients with endolymphatic hydrops and drop attacks.

Evidence for early loading of osseointegrated implants for bone conduction at 4 weeks.

Malleus neck-anchoring malleostapedotomy: preliminary results.

Spatial overlap of combined electroacoustic stimulation determines the electrically evoked response in the guinea pig cochlea.

Fibroblast colonization of modified silicone films.

Reprogramming of single-cell-derived mesenchymal stem cells into hair cell-like cells.

Surgical rehabilitation of paralytic lagophthalmus by platinum chain lid loading: focusing on patient benefit and health-related quality of life.

Cochlear implantation via the middle fossa approach: surgical and programming considerations.

Healing large traumatic eardrum perforations in humans using fibroblast growth factor applied directly or via gelfoam.

Association of benign intracranial hypertension and spontaneous encephalocele with cerebrospinal fluid leak.

Effect of cochlear window fixation on air- and bone-conduction thresholds.

Year: 2012 Volume: 33 Issue: 8

Year: 2012 Volume: 33 Issue: 7

Year: 2012 Volume: 33 Issue: 6

Year: 2012 Volume: 33 Issue: 5

Year: 2012 Volume: 33 Issue: 4

Year: 2012 Volume: 33 Issue: 3

Year: 2012 Volume: 33 Issue: 2

Year: 2012 Volume: 33 Issue: 1

Year: 2011 Volume: 32

Year: 2010 Volume: 31

Year: 2009 Volume: 30

Year: 2008 Volume: 29

Year: 2007 Volume: 28

Year: 2006 Volume: 27

Year: 2005 Volume: 26

Year: 2003 Volume: 24

Activation of PDGFR and EGFR promotes the acquisition of a stem cell-like phenotype in schwannomas.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yi, David Kuo, Selena Z. Zheng, Hao Abhold, Eric L. Brown, Carrie Maiorana Doherty, Joni K. Wang-Rodriguez, Jessica Harris, Jeffery P. Ongkeko, Weg M.
Description	Country affiliation: United States Author Affiliation: Yi D ( Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of California, San Diego, California, USA.)
Abstract	OBJECTIVES: Vestibular schwannomas (VS) are benign tumors that arise from unregulated growth of Schwann cells. Both benign and malignant tumors are believed to contain tumor stem cells that are hypothesized to originate from dysregulation of tumor suppressors and oncogenes. We aimed to determine if schwannoma cells express stem cell genes and markers and if activation of the proto-oncogenes epidermal growth factor receptor and platelet-derived growth factor receptor would regulate the stem cell properties of these cells. METHODS: Immunohistochemical staining was used to determine the expression of stem cell genes in archived VS tissue, immunofluorescence was used to investigate the expression in cell lines, and Western blot analysis was used to measure PDGFR expression in vestibular schwannoma tissue. Upon activation of PDGFR or EGFR in schwannoma cell lines using specific ligands, flow cytometry was used to quantify the side population (SP), stem cell genes were measured using quantitative PCR, and tumorsphere-forming ability was determined. RESULTS: Stem cell genes are expressed in vestibular schwannoma tissue and schwannoma cell lines. Activation of both EGFR and PDGFR resulted in increase in the induction of the expression of the stem cell genes Oct-4 and Nanog and marked increase in tumorsphere-forming ability, but only PDGFR activation resulted in an increase in the side population in JS1 cells. CONCLUSION: Dysregulation of EGFR and PDGFR promotes the acquisition of a stem cell-like phenotype in schwannnoma cells that may be critical in vestibular schwannoma tumorigenesis.
File Format	HTM / HTML
ISSN	15317129
Issue Number	9
Volume Number	33
e-ISSN	15374505
Journal	Otology & Neurotology
Language	English
Publisher	Lippincott Williams & Wilkins
Publisher Date	2012-12-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Immunohistochemistry Neuroma, Acoustic Octamer Transcription Factor-3 Research Support, Non-u.s. Gov't S100 Proteins Peptides Biosynthesis Journal Article Receptors, Platelet-derived Growth Factor Biotransformation Genetics Physiology Antigens, Cd Discipline Otolaryngology Rats Real-time Polymerase Chain Reaction Neoplastic Stem Cells Glycoproteins Metabolism Blotting, Western Pathology Phenotype Animals Receptor Protein-tyrosine Kinases Fluorescent Antibody Technique Cell Line, Tumor Transcription Factors Receptor, Epidermal Growth Factor
Content Type	Text
Resource Type	Article
Subject	Otorhinolaryngology Sensory Systems Neurology (clinical)

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in