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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Toyoizumi, Tomoyasu Ohta, Ryo Nakagawa, Yuzuki Tazura, Yoshiyuki Kuwagata, Makiko Noguchi, Satoshi Yamakage, Kohji |
| Description | Country affiliation: Japan Author Affiliation: Toyoizumi T ( Food and Drug Safety Center, Hadano, Kanagawa, Japan. tomoyasu_toyo@yahoo.co.jp) |
| Abstract | The aim of the present study was to evaluate both sensitivity and specificity of an in vivo skin comet assay using chemically treated, hairless mouse dorsal skin as a model. N-methyl-N'-nitro-N-nitrosoguanidine (MNNG, 0.0125-0.2%), 4-nitroquinoline-1-oxide (4NQO, 0.01-0.25%), mitomycin C (MMC, 0.0125-0.05%), benzo[a]pyrene (B[a]P, 0.25-2%), and 7,12-dimethylbenz[a]anthracene (DMBA, 0.25-1%) were each applied once to the dorsal skin of hairless male mice; after 3h, epidermal skin cells were isolated, and the alkaline comet assay was performed. The assay was performed after 24h for only the B[a]P and DMBA. Furthermore, B[a]P and DMBA were evaluated by alkaline comet assay using liver cells after both 3 and 24h. The mean percent of DNA (%DNA) in tail in the 0.05-0.2% MNNG and 0.1-0.25% 4NQO treatment groups was markedly higher than in the control group at 3h post-application. Although the mean %DNA values in the tail in the B[a]P and DMBA groups were the same as the controls at 3h post-application, the 2% B[a]P and 1% DMBA groups showed significantly higher values versus controls 24h after application. No significant increases in the mean %DNA in the tail were observed in the MMC group. No clear increases in %DNA in the tail were observed in the B[a]P and DMBA groups at 3 or 24h after application in the liver. These results suggest that the in vivo skin comet assay is able to accurately identify DNA-damaging potential with a skin-specific response and is a useful method to detect the DNA-damaging potential of genotoxic chemicals on the skin. |
| File Format | HTM / HTML |
| ISSN | 13835718 |
| e-ISSN | 18793592 |
| Journal | Mutation Research/Genetic Toxicology and Environmental Mutagenesis |
| Issue Number | 2 |
| Volume Number | 726 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-12-24 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Research Support, Non-u.s. Gov't Discipline Genetics Sensitivity And Specificity Toxicity Discipline Biochemistry Mice, Hairless Comet Assay Drug Effects Dna Damage Animals Mutagens Skin Mice Evaluation Studies |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Health, Toxicology and Mutagenesis |
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