| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Rossi, Luis Francisco Luaces, Juan Pablo Browne, Melanie Chirino, Mónica Gabriela Merani, María Susana Mudry, Marta Dolores |
| Description |
Country affiliation: Argentina Author Affiliation: Rossi LF ( Laboratorio de Biología Cromosómica, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, C1121ABG, Piso 10 Lab. 6, Ciudad Autónoma de Buenos Aires, Argentina); Luaces JP ( Laboratorio de Biología Cromosómica, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, C1121ABG, Piso 10 Lab. 6, Ciudad Autónoma de Buenos Aires, Argentina); Browne M ( Laboratorio de Biología Cromosómica, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, C1121ABG, Piso 10 Lab. 6, Ciudad Autónoma de Buenos Aires, Argentina.); Chirino MG ( Laboratorio de Biología Cromosómica, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, C1121ABG, Piso 10 Lab. 6, Ciudad Autónoma de Buenos Aires, Argentina); Merani MS ( Laboratorio de Biología Cromosómica, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, C1121ABG, Piso 10 Lab. 6, Ciudad Autónoma de Buenos Aires, Argentina); Mudry MD ( Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina) |
| Abstract |
Sentinel species are useful tools for studying the deleterious effects of xenobiotics on wildlife. The large hairy armadillo (Chaetophractus villosus) is the most abundant and widely distributed mammal in Argentina. It is a long-lived, omnivorous, burrowing species, with fairly restricted home ranges. To evaluate the level of spontaneous genetic damage in this mammal, we determined the baseline values of several genotoxicity biomarkers. The study included 20 C. villosus adults of both sexes from eight pristine localities within its geographic distribution range. Genotoxicity analysis was performed on 72-h lymphocyte cultures, using mitomycin C as positive control. We obtained the baseline values of mitotic index (MI=10.52±0.30 metaphases/total cells, n=20), chromosome aberrations (CA=0.13±0.22, n=20), sister chromatid exchanges (SCE)=6.55±0.26, n=6) and replication index (RI=1.66, n=6). MI and CA did not show significant differences (P>0.05) among localities or between sexes. No significant differences in MI, CA, SCE, and RI (P>0.05) were found between values from the pristine localities and historical data. There were significant differences in CA, SCE, and RI (P<0.05) between lymphocyte cultures from pristine localities and those exposed to mitomycin C. We propose the large hairy armadillo as a sentinel organism for environmental biomonitoring of genotoxic chemicals due to its abundance, easy manipulation, well-known biology, the fact that it is usually exposed to different mixtures and concentrations of environmental contaminants, and the baseline values of genetic damage characterized by MI, CA, SCE and RI as biomarkers. |
| File Format |
HTM / HTML |
| ISSN |
13835718 |
| e-ISSN |
18793592 |
| Journal |
Mutation Research/Genetic Toxicology and Environmental Mutagenesis |
| Volume Number |
796 |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2016-01-15 |
| Publisher Place |
Netherlands |
| Access Restriction |
Open |
| Subject Keyword |
Research Support, Non-u.s. Gov't Discipline Genetics Mitomycin Toxicity Discipline Biochemistry Metabolism Drug Effects Chemically Induced Dna Damage Mitotic Index Animals Lymphocytes Chromosome Aberrations Sister Chromatid Exchange Xenarthra Xenobiotics |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Genetics Health, Toxicology and Mutagenesis |
