NDLI: Differential Tus-Ter binding and lock formation: implications for DNA replication termination in Escherichia coli.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Moreau, Morgane J. J. Schaeffer, Patrick M.
Description	Country affiliation: Australia Author Affiliation: Moreau MJ ( School of Pharmacy and Molecular Sciences, James Cook University, DB 21, James Cook Drive, Townsville, QLD 4811, Australia.)
Abstract	In E. coli, DNA replication termination occurs at Ter sites and is mediated by Tus. Two clusters of five Ter sites are located on each side of the terminus region and constrain replication forks in a polar manner. The polarity is due to the formation of the Tus-Ter-lock intermediate. Recently, it has been shown that DnaB helicase which unwinds DNA at the replication fork is preferentially stopped at the non-permissive face of a Tus-Ter complex without formation of the Tus-Ter-lock and that fork pausing efficiency is sequence dependent, raising two essential questions: Does the affinity of Tus for the different Ter sites correlate with fork pausing efficiency? Is formation of the Tus-Ter-lock the key factor in fork pausing? The combined use of surface plasmon resonance and GFP-Basta showed that Tus binds strongly to TerA-E and G, moderately to TerH-J and weakly to TerF. Out of these ten Ter sites only two, TerF and H, were not able to form significant Tus-Ter-locks. Finally, Tus's resistance to dissociation from Ter sites and the strength of the Tus-Ter-locks correlate with the differences in fork pausing efficiency observed for the different Ter sites by Duggin and Bell (2009).
File Format	HTM / HTML
ISSN	1742206X
Issue Number	10
Volume Number	8
e-ISSN	17422051
Journal	Molecular BioSystems
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2012-10-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Molecular Biology Discipline Biochemistry Dna Replication Dna, Bacterial Genetics Dna-binding Proteins Dnab Helicases Escherichia Coli Proteins Escherichia Coli Base Sequence Metabolism Molecular Sequence Data Mutation Protein Binding Recombinant Proteins Surface Plasmon Resonance Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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