NDLI: Design of protein congeners containing ß-cyclopropylalanine.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Acevedo-Rocha, Carlos G. Geiermann, Anna-Skrollan Budisa, Nediljko Merkel, Lars
Description	Country affiliation: Germany Author Affiliation: Acevedo-Rocha CG ( Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim, Germany. acevedor@kofo.mpg.de)
Abstract	The non-canonical amino acid (ncAA) analogue of methionine (Met), ß-cyclopropylalanine (Cpa), was successfully incorporated into recombinant proteins expressed in Escherichia coli in a residue-specific manner. Proteins substituted in this way are congeners because they derive from the same gene sequence as the parent protein but contain a fraction of ncAAs. We have expressed congeners using parent and mutant gene sequences of various proteins (lipase, annexin A5, enhanced green fluorescent protein, and barstar) and found that Cpa incorporation is highly dependent on the protein sequence composition. These results indicate that the global amino acid composition of proteins might be a crucial parameter that influences the outcome of unnatural translation. In addition, we could also demonstrate that the chemical nature of the second residue could be essential for successful ncAA incorporation.
File Format	HTM / HTML
ISSN	1742206X
Issue Number	10
Volume Number	8
e-ISSN	17422051
Journal	Molecular BioSystems
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2012-10-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Molecular Biology Discipline Biochemistry Alanine Analogs & Derivatives Bacterial Proteins Biosynthesis Escherichia Coli Metabolism Green Fluorescent Proteins Genetics Amino Acid Sequence Annexin A5 Chemistry Ethers, Cyclic Lipase Mass Spectrometry Methionine Molecular Mimicry Molecular Sequence Data Peptide Chain Initiation, Translational Recombinant Proteins Spectrometry, Fluorescence Journal Article
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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