NDLI: Fluctuation of multiple metabolic pathways is required for Escherichia coli in response to chlortetracycline stress.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lin, Xiangmin Kang, Liqun Li, Hui Peng, Xuanxian
Description	Author Affiliation: Lin X ( Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring (School of Life Sciences, Fujian Agriculture and Forestry University), Fuzhou 35002, People's Republic of China.)
Abstract	Bacterial antibiotic resistance has become a worldwide challenge with the overuse and misuse of drugs. Several mechanisms for the resistance are revealed, but information regarding the bacterial global response to antibiotics is largely absent. In this study, we characterized the differential proteome of Escherichia coli K12 BW25113 in response to chlortetracycline stress using isobaric tags for relative and absolute quantitation labeling quantitative proteomics technology. A total of 723 proteins including 10,763 peptides were identified with 184 decreasing and 147 increasing in abundance by liquid chromatography matrix assisted laser desorption ionization mass spectrometry. Most interestingly, crucial metabolic pathways such as the tricarboxylic acid cycle, pyruvate metabolism and glycolysis/gluconeogenesis sharply fluctuated, while the ribosome protein complexes contributing to the translation process were generally elevated in chlortetracycline stress, which is known for a compensative tactic due to the action of chlortetracycline on the ribosome. Further antimicrobial susceptibility assays validated the role of differential proteins in metabolic pathways using genetically modified mutants of gene deletion of these differential proteins. Our study demonstrated that the down-regulation of metabolic pathways was a part of the global response and played an important role in the antibiotics resistance. These results indicate that reverting of these fluctuated pathways may become a novel strategy to combat antibiotic-resistant bacteria.
File Format	HTM / HTML
ISSN	1742206X
Issue Number	4
Volume Number	10
e-ISSN	17422051
Journal	Molecular BioSystems
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2014-04-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Molecular Biology Discipline Biochemistry Anti-bacterial Agents Pharmacology Chlortetracycline Escherichia Coli Metabolism Metabolic Networks And Pathways Drug Effects Ribosomes Chromatography, Liquid Citric Acid Cycle Down-regulation Gene Expression Profiling Gluconeogenesis Glycolysis Protein Biosynthesis Pyruvic Acid Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization Tetracycline Resistance Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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