NDLI: A lipidomics investigation of the induced hypoxia stress on HeLa cells by using MS and NMR techniques.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yu, Yang Vidalino, Laura Anesi, Andrea Macchi, Paolo Guella, Graziano
Description	Country affiliation: Italy Author Affiliation: Yu Y ( Bioorganic Chemistry Laboratory, Department of Physics, University of Trento, Trento, Italy. graziano.guella@unitn.it.)
Abstract	Induced hypoxia stress on cervical cancer derived cells (HeLa cells) leads to significant changes in their membrane lipid profiles. The lipidome of HeLa cells was characterized by a joint approach wherein liquid chromatography-mass spectrometry (LC-MS) analysis was followed by high resolution NMR measurements. Multivariate data analysis showed apparent separation between control and hypoxia-treated HeLa cells and thus demonstrated hypoxia effects on lipid metabolism. The most striking finding was that hypoxia stimulation significantly reduced the total amount of cellular phosphoinositols (PI) but caused a prominent increase in the amount of lyso phosphocholines (lyso-PC) and lyso phosphoethanolamines (lyso-PE). The observed decrease of PI amount under hypoxic conditions is probably due to the accumulation of cellular myo-inositol, which is known to play a critical role in de novo synthesis of PI. Moreover, our study suggests that polyunsaturated phospholipid species are stronger biomarkers for discriminating the effect of hypoxia treatment. The evaluation of changes in the average unsaturation index (UI) of the membrane lipids acyl chains reveals that UI slightly increases in several lipid classes, thus affecting membrane fluidity and further membrane-dependent functions. The plausible mechanisms by which HeLa cells adapt to hypoxia conditions are also briefly reported.
File Format	HTM / HTML
ISSN	1742206X
Issue Number	4
Volume Number	10
e-ISSN	17422051
Journal	Molecular BioSystems
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2014-04-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Molecular Biology Discipline Biochemistry Cell Hypoxia Physiology Magnetic Resonance Imaging Methods Mass Spectrometry Membrane Lipids Chemistry Stress, Physiological Biological Markers Cell Line, Tumor Chromatography, Liquid Cobalt Hela Cells Humans Lipid Metabolism Lysophosphatidylcholines Metabolism Lysophospholipids Phosphatidylinositols Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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