NDLI: Configurational changes of heme followed by cytochrome c folding reaction.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Choi, Jungkweon Cho, Dae Won Tojo, Sachiko Fujitsuka, Mamoru Majima, Tetsuro
Description	Author Affiliation: Choi J ( The Institute of Scientific and Industrial Research (SANKEN), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan. jkchoi@sanken.osaka-u.ac.jp majima@sanken.osaka-u.ac.jp.)
Abstract	Although the folding kinetics of cytochrome c (Cyt-c), ferric or ferrous Cyt-c, has been extensively investigated as a paradigm for a protein folding reaction using various time-resolved spectroscopic techniques, the configurational change of heme associated with the folding reaction from a ferric Cyt-c to a ferrous Cyt-c induced by one-electron reduction has not been elucidated. To address this issue, we investigated the configurational change of heme in the Cyt-c folding process induced by one-electron reduction using a combination of time-resolved resonance Raman spectroscopy and pulse radiolysis. The results presented herein reveal that the reduction of ferric Cyt-c and the ligation of Met80 occur simultaneously within a timescale of approximately 2 µs, and that the ligand binding and exchange of heme depend on the initial configuration of the heme. The rapid ligation of Met80 observed in this study may be attributed to the intramolecular diffusion of Met80 into ferrous Cyt-c with a 5-coordinated high-spin configuration. Conversely, the ligand exchange of a ferrous Cyt-c with a 6-coordinated low-spin configuration was significantly slower.
File Format	HTM / HTML
ISSN	1742206X
Issue Number	1
Journal	Molecular BioSystems
Volume Number	11
e-ISSN	17422051
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2015-01-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Molecular Biology Discipline Biochemistry Cytochromes c Chemistry Heme Models, Molecular Molecular Conformation Protein Folding Spectrum Analysis, Raman Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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