NDLI: Molecular diversity and phylogeny of Hantaan virus in Guizhou, China: evidence for Guizhou as a radiation center of the present Hantaan virus.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zou, Yang Hu, Jing Wang, Zhao-Xiao Wang, Ding-Ming Li, Ming-Hui Ren, Guo-Dong Duan, Zheng-Xiu Fu, Zhen F. Plyusnin, Alexander Zhang, Yong-Zhen
Spatial Coverage	China
Description	Author Affiliation: Zou Y ( Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping Liuzi 5, 102206 Beijing, PR China.)
Abstract	To gain further insight into the molecular epidemiology of Hantaan virus (HTNV) in Guizhou, China, rodents were captured in this region in 2004 and 2005. In addition, serum samples were collected from four patients. Ten hantaviruses were isolated successfully in cell culture from four humans, two Apodemus agrarius, three Rattus norvegicus and one Rattus nitidus. The nucleotide sequences for their small (S), medium (M) and partial large (L) segments were determined. Phylogenetic analysis of the S and M segment sequences revealed that all of these isolates belong to the species HTNV, suggesting a spillover of HTNV from A. agrarius to Rattus rats. All available isolates from Guizhou were divided into four distinct groups either in the S segment tree or in the M segment tree. The clustering pattern of these isolates in the S segment tree was not in agreement with that in the M or L segment tree, showing that genetic reassortment between HTNV had occurred naturally. Analysis of the S segment sequences from available HTNV strains indicated that they formed three clades. The first clade, which comprised only viruses from Guizhou, was the outgroup of clades II and III. The viruses in the second clade were found in Guizhou and mainly in the far-east Asian region, including China. However, the viruses in the third clade were found in most areas of China, including Guizhou, in which haemorrhagic fever with renal syndrome (HFRS) is endemic. Our results reveal that the highest genetic diversity of HTNV is in a limited geographical region of Guizhou, and suggest that Guizhou might be a radiation centre of the present form of HTNV.
File Format	HTM / HTML
ISSN	00221317
Issue Number	Pt 8
Volume Number	89
e-ISSN	14652099
Journal	Journal of General Virology
Language	English
Publisher	Microbiology Society
Publisher Date	2008-08-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Research Support, Non-u.s. Gov't Humans Lung Molecular Sequence Data Phylogeny Epidemiology Journal Article Genetic Variation Cercopithecus Aethiops China Classification Genetics Rodent Diseases Vero Cells Amino Acid Sequence Hemorrhagic Fever With Renal Syndrome Discipline Virology Hantaan Virus Isolation & Purification Reverse Transcriptase Polymerase Chain Reaction Murinae Sequence Analysis, Dna Virology Animals Molecular Epidemiology Veterinary
Content Type	Text
Resource Type	Article
Subject	Virology

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