NDLI: Initiation of hepatitis B virus genome replication and production of infectious virus following delivery in HepG2 cells by novel recombinant baculovirus vector.

Please wait, while we are loading the content...

Tomato spotted wilt virus glycoproteins induce the formation of endoplasmic reticulum- and Golgi-derived pleomorphic membrane structures in plant cells.

Initiation of hepatitis B virus genome replication and production of infectious virus following delivery in HepG2 cells by novel recombinant baculovirus vector.

D2: major subgenotype of hepatitis B virus in Russia and the Baltic region.

Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4.

Conserved archetypal configuration of the transcriptional control region during the course of BK polyomavirus evolution.

Protective immunity against porcine circovirus 2 by vaccination with ORF2-based DNA and subunit vaccines in mice.

Identification and characterization of a novel envelope protein in Rana grylio virus.

Functional analysis of the putative antiapoptotic genes, p49 and iap4, of Spodoptera litura nucleopolyhedrovirus with RNAi.

The Npro product of classical swine fever virus interacts with IkappaBalpha, the NF-kappaB inhibitor.

Differential reactivity of putative genotype 2 hepatitis C virus F protein between chronic and recovered infections.

Genetic variation of St. Louis encephalitis virus.

A comparative cell biological analysis reveals only limited functional homology between the NS5A proteins of hepatitis C virus and GB virus B.

Distinctive Pattern of Sequence Polymorphism in the NS3 Protein of Hepatitis C Virus Type 1b Reflects Conflicting Evolutionary Pressures

Japanese encephalitis virus infection activates caspase-8 and -9 in a FADD-independent and mitochondrion-dependent manner.

Restricted Semliki Forest virus replication in perforin and Fas-ligand double-deficient mice.

A226V mutation in virus during the 2007 chikungunya outbreak in Kerala, India.

An enterovirus strain isolated from diabetic child belongs to a genetic subcluster of echovirus 11, but is also neutralised with monotypic antisera to coxsackievirus A9.

Severe acute respiratory syndrome coronavirus 3a protein activates the mitochondrial death pathway through p38 MAP kinase activation.

Detection of four genetic subgroup-specific antibodies to human metapneumovirus attachment (G) protein in human serum.

Role of retinoic acid inducible gene-I in human metapneumovirus-induced cellular signalling.

Molecular diversity and phylogeny of Hantaan virus in Guizhou, China: evidence for Guizhou as a radiation center of the present Hantaan virus.

Susceptibility of North American big brown bats (Eptesicus fuscus) to infection with European bat lyssavirus type 1.

Binding of equine infectious anemia virus to the equine lentivirus receptor-1 is mediated by complex discontinuous sequences in the viral envelope gp90 protein.

Field evaluation of a gag/env heteroduplex mobility assay for genetic subtyping of small-ruminant lentiviruses.

Disassembly of African cassava mosaic virus.

Small deletions in the potato leafroll virus readthrough protein affect particle morphology, aphid transmission, virus movement and accumulation.

HC-Pro protein of sugar cane mosaic virus interacts specifically with maize ferredoxin-5 in vitro and in planta.

Delivery of single-chain antibodies (scFvs) directed against the 37/67 kDa laminin receptor into mice via recombinant adeno-associated viral vectors for prion disease gene therapy.

Year: 2008 Volume: 89 Issue: Part 7

Year: 2008 Volume: 89 Issue: Part 6

Year: 2008 Volume: 89 Issue: Part 5

Year: 2008 Volume: 89 Issue: Part 4

Year: 2008 Volume: 89 Issue: Part 3

Year: 2008 Volume: 89 Issue: Part 2

Year: 2008 Volume: 89 Issue: Part 1

Year: 2007 Volume: 88

Year: 2006 Volume: 87

Year: 2005 Volume: 86

Year: 2004 Volume: 85

Year: 2001 Volume: 82

Year: 1997 Volume: 78 Pt 3

Initiation of hepatitis B virus genome replication and production of infectious virus following delivery in HepG2 cells by novel recombinant baculovirus vector.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lucifora, J. Durantel, D. Belloni, L. Barraud, L. Villet, S. Vincent, I. E. Margeridon-Thermet, S. Hantz, O. Kay, A. Levrero, M. Zoulim, F.
Description	Country affiliation: France Author Affiliation: Lucifora J ( INSERM, U871, 151 Cours Albert Thomas, 69003 Lyon, France.)
Abstract	One of the major problems in gaining further insight into hepatitis B virus (HBV)/host-cell interactions is to improve the existing cellular models for the study of HBV replication. The first objective of this study was to improve the system based on transduction of HepG2 cells with a recombinant baculovirus to study HBV replication. A new HBV recombinant baculovirus, Bac-HBV-1.1, in which the synthesis of pre-genomic RNA is driven by a strong mammalian promoter, was generated. Transduction with this new recombinant baculovirus led to higher levels of HBV replication in HepG2 cells compared with levels obtained with previously described baculovirus vectors. The initiation of a complete HBV DNA replication cycle in Bac-HBV-1.1-transduced HepG2 cells was shown by the presence of HBV replicative intermediates, including covalently closed circular DNA (cccDNA). Only low levels of cccDNA were detected in the nucleus of infected cells. Data showed that cccDNA resulted from the recycling of newly synthesized nucleocapsids and was bound to acetylated histones in a chromatin-like structure. HBV particles released into the supernatant of transduced HepG2 cells were infectious in differentiated HepaRG cells. Several Bac-HBV-1.1 baculoviruses containing HBV strains carrying mutations conferring resistance to lamivudine and/or adefovir were constructed. Phenotypic analysis of these mutants confirmed the results obtained with the transfection procedures. In conclusion, an improved cell-culture system was established for the transduction of replication-competent HBV genomes. This will be useful for future studies of the fitness of HBV mutants.
File Format	HTM / HTML
ISSN	00221317
Issue Number	Pt 8
Volume Number	89
e-ISSN	14652099
Journal	Journal of General Virology
Language	English
Publisher	Microbiology Society
Publisher Date	2008-08-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Genome, Viral Research Support, Non-u.s. Gov't Transduction, Genetic Hepatitis B Virus Discipline Virology Humans Dna Replication Metabolism Journal Article Virology Pathogenicity Baculoviridae Hepatocytes Virus Replication Virion Recombination, Genetic Cell Line, Tumor Genetics Physiology Genetic Vectors Evaluation Studies Methods
Content Type	Text
Resource Type	Article
Subject	Virology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Initiation of hepatitis B virus genome replication and production of infectious virus following delivery in HepG2 cells by novel recombinant baculovirus vector.

Human endogenous retrovirus HERV-K113 is capable of producing intact viral particles.

Hepatitis B Virus (HBV) Virion and Covalently Closed Circular DNA Formation in Primary Tupaia Hepatocytes and Human Hepatoma Cell Lines upon HBV Genome Transduction with Replication-Defective Adenovirus Vectors

Transduction of vertebrate cells with recombinant baculovirus.

Effects of Non-Virion Gene Expression Level and Viral Genome Length on the Replication and Pathogenicity of Viral Hemorrhagic Septicemia Virus

Detection of the hepatitis B virus (HBV) covalently-closed-circular DNA (cccDNA) in mice transduced with a recombinant AAV-HBV vector

Introduction of replication-competent hepatitis C virus transcripts using a tetracycline-regulable baculovirus delivery system.

The variable regions of hepatitis C virus glycoprotein E2 have an essential structural role in glycoprotein assembly and virion infectivity.

Optimizing the baculovirus expression vector system.

Initiation of hepatitis B virus genome replication and production of infectious virus following delivery in HepG2 cells by novel recombinant baculovirus vector.

Similar Documents

Initiation of hepatitis B virus genome replication and production of infectious virus following delivery in HepG2 cells by novel recombinant baculovirus vector.

Human endogenous retrovirus HERV-K113 is capable of producing intact viral particles.

Hepatitis B Virus (HBV) Virion and Covalently Closed Circular DNA Formation in Primary Tupaia Hepatocytes and Human Hepatoma Cell Lines upon HBV Genome Transduction with Replication-Defective Adenovirus Vectors

Transduction of vertebrate cells with recombinant baculovirus.

Effects of Non-Virion Gene Expression Level and Viral Genome Length on the Replication and Pathogenicity of Viral Hemorrhagic Septicemia Virus

Detection of the hepatitis B virus (HBV) covalently-closed-circular DNA (cccDNA) in mice transduced with a recombinant AAV-HBV vector

Introduction of replication-competent hepatitis C virus transcripts using a tetracycline-regulable baculovirus delivery system.

The variable regions of hepatitis C virus glycoprotein E2 have an essential structural role in glycoprotein assembly and virion infectivity.

Optimizing the baculovirus expression vector system.

Initiation of hepatitis B virus genome replication and production of infectious virus following delivery in HepG2 cells by novel recombinant baculovirus vector.