NDLI: Structural analysis of a Synechococcus myovirus S-CAM4 and infected cells by atomic force microscopy.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kuznetsov, Yuri G. Martiny, Jennifer B. H. McPherson, Alexander
Spatial Coverage	California
Description	Country affiliation: United States Author Affiliation: Kuznetsov YG ( Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697-3900, USA.)
Abstract	A tailed cyanophage, S-CAM4 (family Myoviridae) from California coastal waters that infects Synechococcus, was characterized by atomic force microscopy. Capsomeric clusters of protein composing the 85 nm diameter icosahedral head were resolved and indicated a triangulation number of T=16. The 140 nm tail assembly, exhibiting a helical appearance with a 13 nm pitch, was seen in both extended and contracted states, the latter exposing the injection tube within. Attached below the base plate were six 50 nm long fibres, and six fibres 275-300 nm in length protruded from the periphery of the base plate. Protein-free DNA was abundant from ruptured heads. Virus attached en masse, in clusters and individually to cells, and cell fragments were recorded, as were perforated cells lysed by the phages. The capsid structure appears most closely related to that of the cyanophage Syn9 and the Bacillus subtilis phage SPO1, which may, in turn, be evolutionarily related to herpesvirus.
File Format	HTM / HTML
ISSN	00221317
Issue Number	Pt 12
Volume Number	91
e-ISSN	14652099
Journal	Journal of General Virology
Language	English
Publisher	Microbiology Society
Publisher Date	2010-12-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Bacteriophages Discipline Virology Isolation & Purification Synechococcus Dna, Viral California Myoviridae Journal Article Virology Virion Ultrastructure Bacteriolysis Virus Attachment Microscopy, Atomic Force Capsid Seawater
Content Type	Text
Resource Type	Article
Subject	Virology

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