NDLI: Rinderpest virus expressing enhanced green fluorescent protein as a separate transcription unit retains pathogenicity for cattle.

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Rinderpest virus expressing enhanced green fluorescent protein as a separate transcription unit retains pathogenicity for cattle.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Banyard, Ashley C. Simpson, Jenny Monaghan, Paul Barrett, Thomas
Description	Country affiliation: United kingdom Author Affiliation: Banyard AC ( Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, UK. a.banyard@vla.defra.gsi.gov.uk)
Abstract	A full-length DNA clone of a virulent strain of rinderpest virus was constructed with the gene for the enhanced green fluorescent protein (eGFP) inserted as a separate transcription unit between the P and M genes. Rescue of the virus from the modified clone using reverse genetics generated a virus that grew to the same levels as the virus rescued from the unmodified DNA clone in cell culture. The recombinant virus expressed eGFP to a high level and was used to follow virus replication in real-time using live-cell imaging. Cattle infected with both the recombinant wild-type virus and the recombinant eGFP expressing virus developed clinical disease similar to that of the wild-type natural virus isolate. Detection of virus in circulating peripheral blood leukocytes was equivalent to that of the animals infected with the wild-type virus. The high level of expression of soluble eGFP by this virus allowed us to detect viral replication in infected animals by confocal microscopy. Imaging vibrating microtome sections by confocal microscopy provided good preservation of tissue and cellular architecture as well as revealing the sites of replication of the virus in different tissues of infected animals.
File Format	HTM / HTML
ISSN	00221317
Issue Number	Pt 12
Volume Number	91
e-ISSN	14652099
Journal	Journal of General Virology
Language	English
Publisher	Microbiology Society
Publisher Date	2010-12-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Green Fluorescent Proteins Research Support, Non-u.s. Gov't Staining And Labeling Virulence Rinderpest Virus Leukocytes Rinderpest Image Processing, Computer-assisted Journal Article Cercopithecus Aethiops Cattle Genetics Transcription, Genetic Genes, Reporter Cell Line Discipline Virology Metabolism Cattle Diseases Virology Pathogenicity Pathology Microscopy, Confocal Growth & Development Animals Methods
Content Type	Text
Resource Type	Article
Subject	Virology

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Rinderpest virus expressing enhanced green fluorescent protein as a separate transcription unit retains pathogenicity for cattle.

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Rinderpest virus expressing enhanced green fluorescent protein as a separate transcription unit retains pathogenicity for cattle.