NDLI: Cell-type-dependent activities of regulatory regions and E2 proteins derived from carcinogenic and non-carcinogenic human alphapapillomaviruses.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Schenker, Astrid Straub, Elke Iftner, Thomas Stubenrauch, Frank
Description	Country affiliation: Germany Author Affiliation: Schenker A ( University Hospital Tuebingen, Institute for Medical Virology and Epidemiology of Viral Diseases, Division of Experimental Virology, Elfriede-Aulhorn-Str 6, D72076 Tuebingen, Germany.)
Abstract	A large number of studies have revealed that persistent infections with certain human papillomavirus (HPV) types are necessary for the development of invasive cancer of the cervix. Recent studies have shown that not only do the major carcinogenic HPV types 16 and 18 encode E6 and E7 oncoproteins with immortalizing activity but also the very weakly or non-carcinogenic types 53, 66, 70 and 82. Currently, it is unknown whether transcriptional differences exist between these viruses that account for carcinogenicity in vivo. Therefore, we compared for the first time the activities of the upstream regulatory regions (URRs) that drive E6 and E7 expression derived from HPV16, -18, -31, -53, -66, -70 and -82 in the absence and presence of the viral E2 transcriptional regulator. URR activities in the absence of E2 varied widely and were further modulated by the cellular background. The co-expression of homologous E2 proteins resulted in repression of the URRs of only some HPV types and this varied with cell type. Activation by E2 proteins was less cell-type dependent but differed in an HPV-type-dependent manner. However, basal URR activity, repression of the URR by E2 and transcriptional activation by E2 did not correlate with HPV carcinogenicity in vivo. In summary, our data do not support the model that the transcriptional activity of human alphapapillomavirus types correlates with epidemiological risk classification.
File Format	HTM / HTML
ISSN	00221317
Issue Number	Pt 6
Volume Number	94
e-ISSN	14652099
Journal	Journal of General Virology
Language	English
Publisher	Microbiology Society
Publisher Date	2013-06-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Promoter Regions, Genetic Research Support, Non-u.s. Gov't Comparative Study Species Specificity Discipline Virology Regulatory Sequences, Nucleic Acid Humans Transcriptional Activation Viral Envelope Proteins Metabolism Papillomavirus Infections Journal Article Virology Alphapapillomavirus Gene Expression Regulation, Viral Classification Genetics
Content Type	Text
Resource Type	Article
Subject	Virology

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