NDLI: Susceptibility of bone marrow-derived macrophages to influenza virus infection is dependent on macrophage phenotype.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Campbell, Gillian M. Nicol, Marlynne Q. Dransfield, Ian Shaw, Darren J. Nash, Anthony A. Dutia, Bernadette M.
Description	Country affiliation: United kingdom Author Affiliation: Campbell GM ( 1â The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh EH25 9RG, UK.); Nicol MQ ( 1â The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh EH25 9RG, UK.); Dransfield I ( 2â Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, Edinburgh EH16 4TL, UK.); Shaw DJ ( 1â The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh EH25 9RG, UK.); Nash AA ( 1â The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh EH25 9RG, UK.); Dutia BM ( 1â The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh EH25 9RG, UK.)
Abstract	The role of the macrophage in influenza virus infection is complex. Macrophages are critical for resolution of influenza virus infections but implicated in morbidity and mortality in severe infections. They can be infected with influenza virus and consequently macrophage infection is likely to have an impact on the host immune response. Macrophages display a range of functional phenotypes, from the prototypical pro-inflammatory classically activated cell to alternatively activated anti-inflammatory macrophages involved in immune regulation and wound healing. We were interested in how macrophages of different phenotype respond to influenza virus infection and therefore studied the infection of bone marrow-derived macrophages (BMDMs) of classical and alternative phenotype in vitro. Our results show that alternatively activated macrophages are more readily infected and killed by the virus than classically activated. Classically activated BMDMs express the pro-inflammatory markers inducible nitric oxide synthase (iNOS) and TNF- , and TNF- expression was further upregulated following infection. Alternatively activated macrophages express Arginase-1 and CD206; however, following infection, expression of these markers was downregulated whilst expression of iNOS and TNF- was upregulated. Thus, infection can override the anti-inflammatory state of alternatively activated macrophages. Importantly, however, this results in lower levels of pro-inflammatory markers than those produced by classically activated cells. Our results showed that macrophage phenotype affects the inflammatory macrophage response following infection, and indicated that modulating the macrophage phenotype may provide a route to develop novel strategies to prevent and treat influenza virus infection.
File Format	HTM / HTML
ISSN	00221317
Issue Number	10
Volume Number	96
e-ISSN	14652099
Journal	Journal of General Virology
Language	English
Publisher	Microbiology Society
Publisher Date	2015-10-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Research Support, Non-u.s. Gov't Discipline Virology Cell Survival Cells, Cultured Immunophenotyping Mice, 129 Strain Macrophages Journal Article Virology Influenza A Virus Growth & Development Phenotype Host-pathogen Interactions Animals Immunology Female Physiology
Content Type	Text
Resource Type	Article
Subject	Virology

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