NDLI: Enhanced hepatitis B virus (HBV) pre-genomic RNA levels and higher transcription efficiency of defective HBV genomes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kandpal, Manish Samal, Jasmine Biswas, Banhi Negi, Avinash Mishra, Vikash C. Tyagi, Neetu Raina, Vimarsh Vivekanandan, Perumal
Description	Country affiliation: India Author Affiliation: Kandpal M ( 1âKusuma School of Biological Sciences, Indian Institute of Technology Delhi, New Delhi, India.); Samal J ( 1âKusuma School of Biological Sciences, Indian Institute of Technology Delhi, New Delhi, India.); Biswas B ( 1âKusuma School of Biological Sciences, Indian Institute of Technology Delhi, New Delhi, India.); Negi A ( 2âMedanta - The Medicity, Gurgaon, Haryana, India.); Mishra VC ( 3âBharat Stem Cell, Safe Blood Organisation, New Delhi, India.); Tyagi N ( 2âMedanta - The Medicity, Gurgaon, Haryana, India.); Raina V ( 2âMedanta - The Medicity, Gurgaon, Haryana, India 3âBharat Stem Cell, Safe Blood Organisation, New Delhi, India.); Vivekanandan P ( 1âKusuma School of Biological Sciences, Indian Institute of Technology Delhi, New Delhi, India.)
Abstract	Defective hepatitis B virus (dHBV) particles contain genomes corresponding to singly spliced HBV RNA. A limited number of studies show that dHBV is present in all chronically HBV-infected patients. Clinical studies have linked dHBV and dHBV gene products to high virus loads and liver damage. The replication characteristics of dHBV genomes remain poorly understood. We found that the splice donor/acceptor sites critical for the formation of dHBV genomes are conserved across HBV genotypes. We report a novel method to create dHBV constructs from corresponding wild-type (WT) HBV constructs. We assessed the transcriptional characteristics of the dHBV constructs with those of the corresponding WT construct using a cell culture model. Interestingly, dHBV constructs had higher pre-genomic RNA levels, transcription efficiency, HBV e antigen levels and intracellular HBV core antigen levels compared with the corresponding WT HBV constructs. Our findings highlight previously unrecognized fundamental molecular characteristics of dHBV genomes and their potential role in the pathogenesis of HBV infection.
File Format	HTM / HTML
ISSN	00221317
Issue Number	10
Volume Number	96
e-ISSN	14652099
Journal	Journal of General Virology
Language	English
Publisher	Microbiology Society
Publisher Date	2015-10-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Cell Line Research Support, Non-u.s. Gov't Defective Viruses Hepatitis B Virus Discipline Virology Humans Biosynthesis Rna, Viral Journal Article Virology Antigens, Viral Hepatocytes Genetics Transcription, Genetic
Content Type	Text
Resource Type	Article
Subject	Virology

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