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A 21-locus autosomal SNP multiplex and its application in forensic science.

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A 21-locus autosomal SNP multiplex and its application in forensic science.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hou, Guangwei Jiang, Xianhua Yang, Yanyan Jia, Fei Li, Qiang Zhao, Jinling Guo, Fei Liu, Limin
Spatial Coverage	China
Description	Country affiliation: China Author Affiliation: Hou G ( Department of Forensic DNA, Criminal Science and Technology Institute of Liaoning province, Shenyang, 110032, China.)
Abstract	To develop a cost-effective technique for single-nucleotide polymorphism (SNP) genotyping and improve the efficiency to analyze degraded DNA, we have established a novel multiplex system including 21-locus autosomal SNPs and amelogenin locus, which was based on allele-specific amplification (ASA) and universal reporter primers (URP). The target amplicons for each of the 21 SNPs arranged from 63 base pair (bp) to 192 bp. The system was tested in 539 samples from three ethnic groups (Han, Mongolian, and Zhuang population) in China, and the total power of discrimination (TPD) and cumulative probability of exclusion (CPE) were more than 0.99999999 and 0.98, respectively. The system was further validated with forensic samples and full profiles could be achieved from degraded DNA and 63 case-type samples. In summary, the multiplex system offers an effective technique for individual identification of forensic samples and is much more efficient in the analysis of degraded DNA compared with standard STR typing.
File Format	HTM / HTML
ISSN	00221198
Issue Number	1
Volume Number	59
e-ISSN	15564029
Journal	Journal of Forensic Sciences
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2014-01-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Dna Primers Research Support, Non-u.s. Gov't Gene Frequency Humans Genotype Discipline Forensic Sciences Genetic Loci Journal Article Electrophoresis, Capillary Ethnic Groups China Polymerase Chain Reaction Validation Studies Genetics Polymorphism, Single Nucleotide Dna Degradation, Necrotic Amelogenin Dna Fingerprinting Methods
Content Type	Text
Resource Type	Article
Subject	Genetics Pathology and Forensic Medicine

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