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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Preativatanyou, Kanok Sirisup, Nantana Payungporn, Sunchai Poovorawan, Yong Thavara, Usavadee Tawatsin, Apiwat Sungpradit, Sivapong Siriyasatien, Padet |
| Spatial Coverage | Thailand |
| Description | Country affiliation: Thailand Author Affiliation: Preativatanyou K ( Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Rama IV Road, Bangkok 10330, Thailand.) |
| Abstract | Accurate identification of insects collected from death scenes provides not only specific developmental data assisting forensic entomologists to determine the postmortem interval more precisely but also other kinds of forensic evidence. However, morphological identification can be complicated due to the similarity among species, especially in the early larval stages. To simplify and make the species identification more practical and reliable, DNA-based identification is preferentially considered. In this study, we demonstrate the application of partial mitochondrial cytochrome oxidase I (COI) and cytochrome oxidase II (COII) sequences for differentiation of forensically important blowflies in Thailand; Chrysomya megacephala, Chrysomya rufifacies and Lucilia cuprina by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The PCR yields a single 1324bp-sized amplicon in all blowfly specimens, followed by direct DNA sequencing. Taq( )I and VspI predicted from the sequencing data provide different RFLP profiles among these three species. Sequence analysis reveals no significant intraspecific divergence in blowfly specimens captured from different geographical regions in Thailand. Accordingly, neighbor-joining tree using Kimura's 2-parameter model illustrates reciprocal monophyly between species. Thus, these approaches serve as promising tools for molecular identification of these three common forensically important blowfly species in Thailand. |
| File Format | HTM / HTML |
| ISSN | 03790738 |
| Issue Number | 1-3 |
| Volume Number | 202 |
| e-ISSN | 18726283 |
| Journal | Forensic Science International |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-10-10 |
| Publisher Place | Ireland |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Forensic Science Dna, Mitochondrial Research Support, Non-u.s. Gov't Electron Transport Complex Iv Phylogeny Thailand Sequence Analysis, Dna Journal Article Animals Polymorphism, Restriction Fragment Length Polymerase Chain Reaction Genetics Diptera |
| Content Type | Text |
| Resource Type | Article |
| Subject | Law Pathology and Forensic Medicine |
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