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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ubero-Pascal, Nicolás López-Esclapez, Raquel García, María-Dolores Arnaldos, María-Isabel |
| Description | Country affiliation: Spain Author Affiliation: Ubero-Pascal N ( Department of Zoology and Physical Anthropology, Faculty de Biology, University of Murcia, 30100 Murcia, Spain. nubero@um.es) |
| Abstract | A comparative morphological study of preimaginal stages (larvae and pupae) of Calliphora vicina Robineau-Desvoidy, 1830 is presented. The entomological samples came from laboratory colonies bred under controlled environmental conditions (25°C and 60% relative humidity). In this study, a recently published technique to clear Diptera larvae for light microscopy and a standard protocol for scanning electron microscopy were used. For the morphological comparison of larval instars I, II and III, and pupae of C. vicina, different larval regions (cephalic, thoracic and abdominal, including anal division), as well the internal chitinised cephalopharyngeal skeleton, were considered separately. Our results focus on showing the changes observed throughout development for the most important structures in the cephalic region (sensilla of maxillary palpus, antennae and oral ridges), the thoracic region (the first segment and its anterior spinose band) and in the anal division of the abdominal region (posterior spiracles and shape of the papillae). In addition, some morphological structures are described or pictured for the first time, such as the ventral organ and the anterior spiracle of larva I and the antenna sensilla, Keilin's organ and wrinkled area of the anal division of all instars. The cephalopharyngeal skeleton is an important structure for the taxonomy of Diptera larvae in all instars, including Calliphoridae. Our observations in C. vicina indicate that an in-depth review of the sclerite composition is needed. Pupae and larvae stages can only be compared by following the segmentary spinose bands and the anal segment, where the morphology of the posterior spiracles and papillae can be observed, in some cases despite the reduced condition of the latter. |
| File Format | HTM / HTML |
| ISSN | 03790738 |
| Issue Number | 1-3 |
| Volume Number | 219 |
| e-ISSN | 18726283 |
| Journal | Forensic Science International |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-06-10 |
| Publisher Place | Ireland |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Pupa Discipline Forensic Science Growth & Development Research Support, Non-u.s. Gov't Microscopy, Electron, Scanning Animals Larva Microscopy Ultrastructure Light Diptera Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Law Pathology and Forensic Medicine |
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