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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Rocchetti, Maria Teresa Papale, Massimo D'apollo, Anna Maria Suriano, Ida Valentina Di Palma, Anna Maria Vocino, Grazia Montemurno, Eustacchio Varraso, Leonarda Grandaliano, Giuseppe Di Paolo, Salvatore Gesualdo, Loreto |
| Description | Country affiliation: Italy Author Affiliation: Rocchetti MT ( Proteomics and Mass Spectrometry Core Facility, Research Center BioAgroMed, University of Foggia, Foggia, Italy.) |
| Abstract | BACKGROUND AND OBJECTIVES: IgA nephropathy has variable clinical presentation and progression. Its definitive diagnosis and prognosis require renal biopsy. The identification of new biomarkers allowing noninvasive diagnosis and monitoring of disease activity would be advantageous. This study analyzed the urine proteome of IgA nephropathy patients at an early stage of disease. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: Urine from 49 IgA nephropathy patients, 42 CKD patients, and 40 healthy individuals was analyzed by surface-enhanced laser desorption/ionization time of flight/mass spectrometry. Differentially excreted proteins were identified by matrix-enhanced laser desorption/ionization time of flight/mass spectrometry, confirmed by immunologic methods, and validated in an independent set of patients (14 IgA nephropathy and 24 CKD). All patients were recruited at the Division of Nephrology of the University of Foggia from January of 2005 to March of 2007. RESULTS: Two proteins, with 21,598 and 23,458 m/z, were significantly decreased in IgA nephropathy and identified as Perlecan laminin G-like 3 peptide and Ig κ light chains, respectively. Western blot analysis confirmed the lower urinary excretion of laminin G-like 3 in IgA nephropathy patients compared with CKD patients and healthy individuals. Immunonephelometry analysis confirmed the lower urinary excretion of free κ light chains in IgA nephropathy patients compared with CKD patients and healthy individuals. Immunohistochemistry analysis justified the urinary excretion profile of such proteins in IgA nephropathy. Finally, urinary free κ light chains and laminin G-like 3 concentration inversely correlated with severity of clinical and histologic features of our IgA nephropathy cohort. CONCLUSIONS: Laminin G-like 3 and free κ light chains can contribute to the noninvasive assessment of IgA nephropathy disease activity. |
| File Format | HTM / HTML |
| ISSN | 15559041 |
| e-ISSN | 1555905X |
| DOI | 10.2215/CJN.05950612 |
| Journal | Clinical Journal of the American Society of Nephrology |
| Issue Number | 7 |
| Volume Number | 8 |
| Language | English |
| Publisher | American Society of Nephrology |
| Publisher Date | 2013-07-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Immunohistochemistry Research Support, Non-u.s. Gov't Peptide Fragments Biological Markers Predictive Value Of Tests Nephelometry And Turbidimetry Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization Case-control Studies Immunoglobulin Kappa-chains Laminin Down-regulation Severity Of Illness Index Urine Glomerulonephritis, Iga Discipline Nephrology Linear Models Blotting, Western Reproducibility Of Results Pathology Biopsy Proteomics Heparan Sulfate Proteoglycans Analysis Of Variance |
| Content Type | Text |
| Resource Type | Article |
| Subject | Transplantation Critical Care and Intensive Care Medicine Nephrology Epidemiology |
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