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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Matamala, Nerea Vargas, María Teresa González-Cámpora, Ricardo Miñambres, Rebeca Arias, José Ignacio Menéndez, Primitiva Andrés-León, Eduardo Gómez-López, Gonzalo Yanowsky, Kira Calvete-Candenas, Julio Inglada-Pérez, Lucía Martínez-Delgado, Beatriz Benítez, Javier |
| Description | Author Affiliation: Matamala N ( Human Cancer Genetics Programme and.); Vargas MT ( Pathology Service and.); González-Cámpora R ( Pathology Service and.); Miñambres R ( Projects Unit, Sistemas Genómicos, Valencia, Spain); Arias JI ( Surgery Service and.); Menéndez P ( Pathology Service, Hospital Monte Naranco, Oviedo, Spain); Andrés-León E ( Bioinformatics Unit, Spanish National Cancer Research Centre (CNIO), Madrid, Spain); Gómez-López G ( Bioinformatics Unit, Spanish National Cancer Research Centre (CNIO), Madrid, Spain); Yanowsky K ( Human Cancer Genetics Programme and.); Calvete-Candenas J ( Medical Oncology Service, Hospital Virgen de la Macarena, Sevilla, Spain); Inglada-Pérez L ( Human Cancer Genetics Programme and Spanish Network in Rare Diseases (CIBERER), Madrid, Spain); Martínez-Delgado B ( Molecular Genetics Unit, Research Institute of Rare Diseases (IIER), Instituto de Salud Carlos III (ISCIII), Madrid, Spain.); Benítez J ( Human Cancer Genetics Programme and Spanish Network in Rare Diseases (CIBERER), Madrid, Spain) |
| Abstract | BACKGROUND: The identification of novel biomarkers for early breast cancer detection would be a great advance. Because of their role in tumorigenesis and stability in body fluids, microRNAs (miRNAs) are emerging as a promising diagnostic tool. Our aim was to identify miRNAs deregulated in breast tumors and evaluate the potential of circulating miRNAs in breast cancer detection. METHODS: We conducted miRNA expression profiling of 1919 human miRNAs in paraffin-embedded tissue from 122 breast tumors and 11 healthy breast tissue samples. Differential expression analysis was performed, and a microarray classifier was generated. The most relevant miRNAs were analyzed in plasma from 26 healthy individuals and 83 patients with breast cancer (36 before and 47 after treatment) and validated in 116 healthy individuals and 114 patients before treatment. RESULTS: We identified a large number of miRNAs deregulated in breast cancer and generated a 25-miRNA microarray classifier that discriminated breast tumors with high diagnostic sensitivity and specificity. Ten miRNAs were selected for further investigation, of which 4 (miR-505-5p, miR-125b-5p, miR-21-5p, and miR-96-5p) were significantly overexpressed in pretreated patients with breast cancer compared with healthy individuals in 2 different series of plasma. MiR-505-5p and miR-96-5p were the most valuable biomarkers (area under the curve 0.72). Moreover, the expression levels of miR-3656, miR-505-5p, and miR-21-5p were decreased in a group of treated patients. CONCLUSIONS: Circulating miRNAs reflect the presence of breast tumors. The identification of deregulated miRNAs in plasma of patients with breast cancer supports the use of circulating miRNAs as a method for early breast cancer detection. |
| File Format | HTM / HTML |
| ISSN | 00099147 |
| e-ISSN | 15308561 |
| Journal | Clinical Chemistry |
| Issue Number | 8 |
| Volume Number | 61 |
| Language | English |
| Publisher | American Association for Clinical Chemistry |
| Publisher Date | 2015-08-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Micrornas Research Support, Non-u.s. Gov't Tumor Markers, Biological Oligonucleotide Array Sequence Analysis Gene Expression Regulation, Neoplastic Reference Values Discipline Laboratory Medicine Breast Neoplasms Blood Reproducibility Of Results Pathology Discipline Clinical Chemistry Early Detection Of Cancer Genetics Multicenter Study |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry (medical) Clinical Biochemistry |
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