| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Chatterjee, M. Von Ungern-sternberg, S. N. I. Seizer, P. Schlegel, F. Büttcher, M. Sindhu, N. A. Müller, S. Mack, A. Gawaz, M. |
| Description |
Country affiliation: Germany Author Affiliation: Chatterjee M ( Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Universität Tübingen, 72076 Tübingen, Germany.); von Ungern-Sternberg SN ( Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Universität Tübingen, 72076 Tübingen, Germany.); Seizer P ( Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Universität Tübingen, 72076 Tübingen, Germany.); Schlegel F ( Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Universität Tübingen, 72076 Tübingen, Germany.); Büttcher M ( Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Universität Tübingen, 72076 Tübingen, Germany.); Sindhu NA ( Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Universität Tübingen, 72076 Tübingen, Germany.); Müller S ( Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Universität Tübingen, 72076 Tübingen, Germany.); Mack A ( Institute of Anatomy, Universität Tübingen, Neuroanatomie, 72074 Tübingen, Germany.); Gawaz M ( Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Universität Tübingen, 72076 Tübingen, Germany.) |
| Abstract |
Platelets store and release CXCL12 (SDF-1), which governs differentiation of hematopoietic progenitors into either endothelial or macrophage-foam cells. CXCL12 ligates CXCR4 and CXCR7 and regulates monocyte/macrophage functions. This study deciphers the relative contribution of CXCR4-CXCR7 in mediating the effects of platelet-derived CXCL12 on monocyte function, survival, and differentiation. CXCL12 and macrophage migration inhibitory factor (MIF) that ligate CXCR4-CXCR7 induced a dynamic bidirectional trafficking of the receptors, causing CXCR4 internalization and CXCR7 externalization during chemotaxis, thereby influencing relative receptor availability, unlike MCP-1. In vivo we found enhanced accumulation of platelets and platelet-macrophage co-aggregates in peritoneal fluid following induction of peritonitis in mice. The relative surface expression of CXCL12, CXCR4, and CXCR7 among infiltrated monocytes was also enhanced as compared with peripheral blood. Platelet-derived CXCL12 from collagen-adherent platelets and recombinant CXCL12 induced monocyte chemotaxis specifically through CXCR4 engagement. Adhesion of monocytes to immobilized CXCL12 and CXCL12-enriched activated platelet surface under static and dynamic arterial flow conditions were mediated primarily through CXCR7 and were counter-regulated by neutralizing platelet-derived CXCL12. Monocytes and culture-derived-M1-M2 macrophages phagocytosed platelets, with the phagocytic potential of culture-derived-M1 macrophages higher than M2 involving CXCR4-CXCR7 participation. CXCR7 was the primary receptor in promoting monocyte survival as exerted by platelet-derived CXCL12 against BH3-mimetic induced apoptosis (phosphatidylserine exposure, caspase-3 activation, loss of mitochondrial transmembrane potential). In co-culture experiments with platelets, monocytes predominantly differentiated into CD163(+) macrophages, which was attenuated upon CXCL12 neutralization and CXCR4/CXCR7 blocking antibodies. Moreover, OxLDL uptake by platelets induced platelet apoptosis, like other platelet agonists TRAP and collagen-related peptide (CRP). CXCL12 facilitated phagocytosis of apoptotic platelets by monocytes and M1-M2 macrophages, also promoted their differentiation into foam cells via CXCR4 and CXCR7. Thus, platelet-derived CXCL12 could regulate monocyte-macrophage functions through differential engagement of CXCR4 and CXCR7, indicating an important role in inflammation at site of platelet accumulation. |
| File Format |
HTM / HTML |
| e-ISSN |
20414889 |
| DOI |
10.1038/cddis.2015.233 |
| Journal |
Cell Death and Disease |
| Volume Number |
6 |
| Language |
English |
| Publisher |
Nature Publishing Group |
| Publisher Date |
2015-11-19 |
| Publisher Place |
Great Britain (UK) |
| Access Restriction |
Open |
| Subject Keyword |
Research Support, Non-u.s. Gov't Cell Survival Blood Platelets Cytology Macrophages Metabolism Blood Discipline Cell Biology Monocytes Immunology Foam Cells Animals Receptors, Cxcr Cell Differentiation Physiology Mice |
| Content Type |
Text |
| Resource Type |
Article |
