NDLI: Inhibition of cystathionine-beta-synthase activity during renal ischemia-reperfusion: role of pH and nitric oxide.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Prathapasinghe, Gamika A. Siow, Yaw L. Xu, Zhibin Karmin, O.
Description	Author Affiliation: Prathapasinghe GA ( Department of Animal Science, University of Manitoba, and Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Research Centre, 351 Tache Ave., Winnipeg, Manitoba, Canada R2H 2A6.)
Abstract	Our recent study (Prathapasinghe GA, Siow YL, O K. Am J Physiol Renal Physiol 292: F1354-F1363, 2007) indicates that homocysteine (Hcy) plays a detrimental role in ischemia-reperfusion-induced renal injury. Elevation of renal Hcy concentration during ischemia-reperfusion is attributed to reduced activity of cystathionine-beta-synthase (CBS) that catalyzes the rate-limiting step in the transsulfuration pathway for the metabolism of the majority of Hcy in the kidney. However, the mechanisms of impaired CBS activity in the kidney are unknown. The aim of this study was to investigate the effects of pH and nitric oxide (NO) on the CBS activity in the kidney during ischemia-reperfusion. The left kidney of a Sprague-Dawley rat was subjected to ischemia-reperfusion. The CBS activity was significantly reduced in kidneys subjected to ischemia alone (15-60 min) or subjected to ischemia followed by reperfusion for 1-24 h. The pH was markedly reduced in kidneys upon ischemia. Injection of alkaline solution into the kidney partially restored the CBS activity during ischemia. Further analysis revealed that reduction of CBS activity during reperfusion was accompanied by an elevation of NO metabolites (nitrate and nitrite) in the kidney tissue. Injection of a NO scavenger, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO), restored the CBS activity in the kidneys subjected to ischemia-reperfusion. Treatment with PTIO could abolish ischemia-reperfusion-induced lipid peroxidation and prevent cell death in the kidney. These results suggested that metabolic acidosis during ischemia and accumulation of NO metabolites during reperfusion contributed, in part, to reduced CBS activity leading to an elevation of renal Hcy levels, which in turn, played a detrimental role in the kidney.
File Format	HTM / HTML
ISSN	1931857X
Issue Number	4
Volume Number	295
e-ISSN	15221466
Journal	AJP: Renal Physiology
Language	English
Publisher	American Physiological Society
Publisher Date	2008-10-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Research Support, Non-u.s. Gov't Enzyme Inhibitors Cystathionine Beta-synthase Oxidative Stress Free Radical Scavengers Male Kidney Antagonists & Inhibitors Journal Article Nitric Oxide Donors Discipline Physiology Cyclic N-oxides Physiology Hydrogen-ion Concentration Reperfusion Injury Nitroprusside Rats Rats, Sprague-dawley Discipline Nephrology Pharmacology Nitric Oxide Metabolism Drug Effects Animals Imidazoles Enzyme Activation Enzymology Acidosis Homocysteine Lipid Peroxidation Ng-nitroarginine Methyl Ester Apoptosis
Content Type	Text
Resource Type	Article
Subject	Physiology Urology

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