NDLI: Topology of Schwann cells and sympathetic innervation along preglomerular vessels: a confocal microscopic study in protein S100B/EGFP transgenic mice.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Darlot, Fannie Artuso, Annie Lautredou-Audouy, Nicole Casellas, Daniel
Description	Country affiliation: France Author Affiliation: Darlot F ( Groupe Rein et Hypertension, IURC, 641 Ave. Doyen Giraud, 34093 Montpellier Cédex 5, France.)
Abstract	Schwann cells (Sc), associated axons, and nearby vascular endothelium constitute a functional trilogy of major importance during the development and regrowth of peripheral vascular nerves. The goal of the present study is to provide a technique of triple fluorescence confocal imaging of these cell types along renal preglomerular vessels. We took advantage of a protein S100B/EGFP transgenic mouse to visualize Sc. The endothelium was labeled with an intravenous injection of fluorescently tagged lectin, and after tissue processing, adrenergic nerves were revealed with an antibody against the marker protein synaptophysin. As a validation step, we found that EGFP-positive perivascular cells with prominent cell bodies and extensive, multidirectional cell processes were protein S100B positive. They were identified as Sc and indirectly assumed to be unmyelinated Sc. By contrast, we found strong EGFP expression in proximal epithelial cells and in the epithelium lining thin limbs of Henle. This epithelial fluorescence was not associated with immunoreactive protein S100B and thus corresponded to ectopic EGFP expressions in this mouse strain. Sc were organized in bundles or as a meshwork surrounding the preglomerular vasculature from arcuate arteries to afferent arterioles. No Sc were detected in the medulla. Although most Sc were closely apposed to adrenergic varicosities, many varicosities were not associated with detectable Sc processes. The present technique, and the capacity of confocal microscopy to yield three-dimensional imaging, allow the study of the microtopology of Sc and related sympathetic axons in the renal perivascular interstitium.
File Format	HTM / HTML
ISSN	1931857X
Issue Number	4
Volume Number	295
e-ISSN	15221466
Journal	AJP: Renal Physiology
Language	English
Publisher	American Physiological Society
Publisher Date	2008-10-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Immunohistochemistry Green Fluorescent Proteins Research Support, Non-u.s. Gov't Schwann Cells S100 Proteins Biological Markers Innervation Cytology Male Journal Article S100 Calcium Binding Protein Beta Subunit Discipline Physiology Plant Lectins Imaging, Three-dimensional Sympathetic Nervous System Genetics Physiology Adrenergic Fibers Discipline Nephrology Mice, Transgenic Microscopy, Confocal Animals Nerve Growth Factors Mice Methods Microscopy, Fluorescence Kidney Glomerulus
Content Type	Text
Resource Type	Article
Subject	Physiology Urology

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