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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Dong, X. Y. Wang, Y. M. Yuan, C. Zou, X. T. |
| Description | Country affiliation: China Author Affiliation: Dong XY ( Feed Science Institute, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China.) |
| Abstract | To better understand the digestive capacity in domestic pigeons (Columba livia), this study was conducted to evaluate nutrient transporters and digestive enzymes gene expression in small intestine and yolk sac membrane (YSM) during pre- and posthatch development. We investigated the oligopeptide transporter Pept1, sodium glucose transporter SGLT1, glucose transporter GLUT2, aminopeptidase-N (APN), and sucrase-isomaltase (SI). Intestine was collected at embryo d 12, 14, and 16, day of hatch, and d 1, 3, 5, 8, and 14 posthatch. The YSM was collected at embryo d 12, 14, 16, and day of hatch. The cDNA fragments for Pept1, SGLT1, GLUT2, APN, and SI were isolated and cloned using reverse-transcription PCR. The sequences data showed that these genes were highly identical to the gene of chicken. The mRNA expression of each gene was assayed using real-time PCR. Expression of intestinal nutrient transporters increased linearly (P<0.001) with age whereas that of APN increased quadratically (P<0.001) and SI increased cubically (P=0.001). Levels of PepT1 mRNA were greatest in the duodenum (P=0.001), GLUT2 and SGLT1 mRNA were greatest in the jejunum (P=0.002) and APN were greatest in the ileum (P=0.004). The YSM expressed all the examined genes. The YSM-expressed genes decreased between embryo d 16 and day of hatch, whereas intestine-expressed genes increased. Our findings provide a comprehensive profile of gene expression patterns of nutrient transporter and digestive enzyme in the small intestine and YSM of pigeons and establish a foundation for future research on the nutrients requirements for young pigeons. |
| File Format | HTM / HTML |
| ISSN | 00325791 |
| e-ISSN | 15253171 |
| Journal | Poultry Science |
| Issue Number | 8 |
| Volume Number | 91 |
| Language | English |
| Publisher | Oxford University Press |
| Publisher Date | 2012-08-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | Open |
| Subject Keyword | Discipline Veterinary Medicine Carrier Proteins Metabolism Columbidae Embryology Digestion Physiology Gene Expression Regulation, Developmental Intestines Enzymology Yolk Sac Aging Animals Cloning, Molecular Gene Expression Regulation, Enzymologic Real-time Polymerase Chain Reaction Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Animal Science and Zoology |
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