NDLI: Reversible adsorption of catalase onto Fe(3+) chelated poly(AAm-GMA)-IDA cryogels.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Aktas Uygun, Deniz Uygun, Murat Akgöl, Sinan Denizli, Adil
Description	Author Affiliation: Aktas Uygun D ( Chemistry Department, Adnan Menderes University, Aydin, Turkey. Electronic address: daktas@adu.edu.tr.); Uygun M ( Koçarli Vocational and Training School, Adnan Menderes University, Aydin, Turkey.); Akgöl S ( Biochemistry Department, Ege University, Izmir, Turkey.); Denizli A ( Chemistry Department, Hacettepe University, Ankara, Turkey.)
Abstract	In this presented study, poly(acrylamide-glycidyl methacrylate) [poly(AAm-GMA)] cryogels were synthesized by cryopolymerization technique at sub-zero temperature. Prepared cryogels were then functionalized with iminodiacetic acid (IDA) and chelated with Fe(3+) ions in order produce the metal chelate affinity matrix. Synthesized cryogels were characterized with FTIR, ESEM and EDX analysis, and it was found that the cryogel had sponge like structure with interconnected pores and their pore diameter was about 200 µm. Fe(3+) chelated poly(AAm-GMA)-IDA cryogels were used for the adsorption of catalase and optimum adsorption conditions were determined by varying the medium pH, initial catalase concentration, temperature and ionic strength. Maximum catalase adsorption onto Fe(3+) chelated poly(AAm-GMA)-IDA cryogel was found to be 12.99 mg/g cryogel at 25 °C, by using pH 5.0 acetate buffer. Adsorbed catalase was removed from the cryogel by using 1.0M of NaCl solution and desorption yield was found to be 96%. Additionally, reusability profile of the Fe(3+) chelated poly(AAm-GMA)-IDA cryogel was also investigated and it was found that, adsorption capacity of the cryogels didn't decrease significantly at the end of the 40 reuses. Catalase activity studies were also tested and it was demonstrated that desorbed catalase retained 70% of its initial activity.
File Format	HTM / HTML
ISSN	09284931
Volume Number	50
e-ISSN	18730191
Journal	Materials Science and Engineering: C
Language	English
Publisher	Elsevier
Publisher Date	2015-05-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Materials Science Acrylic Resins Chemistry Catalase Isolation & Purification Cryogels Imino Acids Iron Chelating Agents Adsorption Animals Cattle Hydrogen-ion Concentration Kinetics Microscopy, Electron, Scanning Osmolar Concentration Recycling Spectrometry, X-ray Emission Spectroscopy, Fourier Transform Infrared Temperature Journal Article
Content Type	Text
Resource Type	Article
Subject	Mechanics of Materials Biomaterials Condensed Matter Physics Bioengineering Mechanical Engineering

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