NDLI: A sensitive DNA biosensor fabricated from gold nanoparticles and graphene oxide on a glassy carbon electrode.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hajihosseini, Saeedeh Nasirizadeh, Navid Hejazi, Mohammad Saeid Yaghmaei, Parichereh
Description	Country affiliation: Iran Author Affiliation: Hajihosseini S ( Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.); Nasirizadeh N ( Department of Textile and Polymer Engineering, Yazd Branch, Islamic Azad University, Yazd, Iran. Electronic address: nasirizadeh@iauyazd.ac.ir.); Hejazi MS ( Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.); Yaghmaei P ( Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.)
Abstract	A sensitive electrochemical DNA biosensor was developed for Helicobacter pylori (H. pylori) detection using differential pulse voltammetry. Single-stranded DNA probe was immobilized on a graphene oxide/gold nanoparticles modified glassy carbon electrode (GO/AuNPs/GCE). A hybridization reaction was conducted with the target DNA and the immobilized DNA on the electrode surface. Oracet blue (OB) was selected for the first time as a redox indicator for amplifying the electrochemical signal of DNA. Enhanced sensitivity was achieved through combining the excellent electric conductivity of GO/AuNPs and the electroactivity of the OB. The DNA biosensor displayed excellent performance to demonstrate the differences between the voltammetric signals of the OB obtained from different hybridization samples (non-complementary, mismatch and complementary DNAs). The proposed biosensor has a linear range of 60.0-600.0 pM and a detection limit of 27.0 pM for detection of H. pylori. In addition, the biosensor have responded very well in the simulated real sample evaluations, signifying its potential to be used in future clinical detection of the H. pylori bacteria.
File Format	HTM / HTML
ISSN	09284931
Volume Number	61
e-ISSN	18730191
Journal	Materials Science and Engineering: C
Language	English
Publisher	Elsevier
Publisher Date	2016-04-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Materials Science Biosensing Techniques Methods Carbon Chemistry Dna, Bacterial Analysis Graphite Helicobacter Pylori Metal Nanoparticles Electrodes Journal Article
Content Type	Text
Resource Type	Article
Subject	Mechanics of Materials Biomaterials Condensed Matter Physics Bioengineering Mechanical Engineering

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