NDLI: Targeted quantitative analysis of superoxide dismutase 1 in cisplatin-sensitive and cisplatin-resistant human ovarian cancer cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kim, Jong Won Nie, Bei Sahm, Heather Brown, Dawn P. G. Tegeler, Tony You, Jin-Sam Wang, Mu
Description	Country affiliation: United States Author Affiliation: Kim JW ( Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.)
Abstract	Protein quantification in a complex protein mixture presents a daunting task in biochemical analysis. Antibody-based immunoassays are traditional methods for protein quantification. However, there are issues associated with accuracy and specificity in these assays, especially when the changes are small (e.g., <2-fold). With recent developments in mass spectrometry, monitoring a selected peptide, thus protein, in a complex biological sample has become possible. In this study, we demonstrate a simple mass spectrometry-based method for selective measurement of a moderately low abundant protein, superoxide dismutase 1 (SOD1), in cisplatin-sensitive and cisplatin-resistant human ovarian cancer cells. Selected-reaction-monitoring (SRM) technology was employed to specifically analyze the target peptides in a pair of human ovarian cancer cell lines: 2008/2008-C13*5.25 (cisplatin-sensitive/cisplatin-resistant, respectively). The observed 1.47-fold higher expression in the resistant cell line is consistent with findings by other approaches. This robust liquid chromatography/mass spectrometry (LC/MS) method provides a powerful tool for targeted proteomic verification and/or validation studies.
File Format	HTM / HTML
ISSN	15700232
e-ISSN	1873376X
DOI	10.1016/j.jchromb.2010.01.013
Journal	Journal of Chromatography B
Issue Number	7-8
Volume Number	878
Language	English
Publisher	Elsevier
Publisher Date	2010-03-01
Publisher Place	Netherlands
Access Restriction	Open
Subject Keyword	Discipline Analytical Chemistry Cisplatin Therapeutic Use Ovarian Neoplasms Drug Therapy Enzymology Proteomics Superoxide Dismutase Cell Line, Tumor Drug Resistance, Neoplasm Enzyme Stability Mass Spectrometry Peptide Fragments Metabolism Reproducibility Of Results Sensitivity And Specificity Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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Targeted Quantitative Analysis of Superoxide Dismutase 1 in Cisplatin-sensitive and Cisplatin-resistant Human Ovarian Cancer Cells

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