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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kubica, Pawel Kot-Wasik, Agata Wasik, Andrzej Namiesnik, Jacek Landowski, Piotr |
| Description | Country affiliation: Poland Author Affiliation: Kubica P ( Department of Analytical Chemistry, Chemical Faculty, Gdansk University of Technology, Gdansk, Poland. pawel.kubica.pg@gmail.com) |
| Abstract | A new analytical procedure was described for the simultaneous determination of lactulose, mannitol and sucrose in urine, in which HILIC chromatography and tandem mass spectrometry detection are used. Sugars are orally administered for the estimation of intestinal permeability in children digestive tract. Samples were purified by dispersive solid phase extraction (d-SPE) using Amberlite MB150 resin. Raffinose was selected as an internal standard. The chosen chromatographic separation was carried out on ZIC(®)-HILIC column in 10 min at a flow rate of 0.3 mL/min, using mixture of acetonitrile (ACN) and ammonium acetate (NH(4)Ac) in water (H(2)O) as the mobile phase. Within-run precision (CV) measured at three concentrations was 1.08%, 0.32% and 0.49% for lactulose; 1.88%, 0.47% and 0.75% for mannitol, 2.95%, 1.31% and 0.6% for sucrose. Between-run CVs were 0.75%, 1.1% and 1.2% for lactulose; 1.1%, 1.02% and 1.01% for mannitol; 1.17%, 1.4% and 1.05% for sucrose. Analytical recovery of all three sugar probes was 95.06-99.92%. The detection limits were: 15.94 ng/mL for lactulose, 17.10 ng/mL for sucrose and 11.48 ng/mL for mannitol. The proposed method is rapid, simple, sensitive and suitable for the determination of intestinal permeability of the sugar derivatives in children. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| Volume Number | 907 |
| e-ISSN | 1873376X |
| Journal | Journal of Chromatography B |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-10-15 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Chromatography, High Pressure Liquid Methods Intestinal Absorption Intestinal Diseases Urine Lactulose Mannitol Sucrose Tandem Mass Spectrometry Calibration Case-control Studies Child Humans Metabolism Administration & Dosage Pharmacokinetics Limit Of Detection Reproducibility Of Results Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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