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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Moreno, Ivo Barroso, Mário Martinho, Ana Cruz, Angelines Gallardo, Eugenia |
| Description | Country affiliation: Portugal Author Affiliation: Moreno I ( CICS-UBI-Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, 6201-556 Covilhã, Portugal.); Barroso M ( Instituto Nacional de Medicina Legal e Ciências Forenses-Delegação do Sul, Rua Manuel Bento de Sousa, 3, 1169-201 Lisboa, Portugal.); Martinho A ( CICS-UBI-Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, 6201-556 Covilhã, Portugal.); Cruz A ( Servicio de Toxicologia Forense-Instituto de Ciencias Forenses Luís Concheiro, Universidad de Santiago de Compostela, San Francisco s/n, 15782 Santiago de Compostela, Spain.); Gallardo E ( CICS-UBI-Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, 6201-556 Covilhã, Portugal. Electronic address: egallardo@fcsaude.ubi.pt.) |
| Abstract | Ketamine is a club drug widely abused for its hallucinogenic effects, being also used as a 'date-rape' drug in recent years. We have developed an analytical method using gas chromatography-tandem mass spectrometry (GC-MS/MS) for the identification and quantification of ketamine and its major metabolite in urine and plasma. No derivatization step is needed to accomplish analysis. The compounds were extracted from 0.25mL of sample using microextraction by packed sorbent on mixed mode (M1) cartridges. Calibration curves were linear in the range of 10-250ng/mL for urine and 10-500ng/mL for plasma, with determination coefficients higher than 0.99. The limit of detection (LOD) was 5ng/mL for both compounds in both specimens. Recoveries ranged from 63 to 101%, while precision and accuracy were below 14% and 15%, respectively. These low limits of detection and the quite high recoveries obtained, in very low sample amounts, allow detecting small quantities of the compounds, making this procedure suitable for those laboratories performing routine analysis in the field of forensic toxicology. Compared with existing methods, the herein described procedure is fast, since no derivatization step is required, and cost effective for the quantification of ketamine and norketamine in biological specimens by gas chromatography. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| Volume Number | 1004 |
| e-ISSN | 1873376X |
| Journal | Journal of Chromatography B |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-11-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Gas Chromatography-mass Spectrometry Methods Ketamine Analogs & Derivatives Blood Urine Tandem Mass Spectrometry Calibration Humans Limit Of Detection Reference Standards Journal Article Validation Studies |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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