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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Penchala, Sujan Dilly Tjia, John El Sherif, Omar Back, David J. Khoo, Saye H. Else, Laura J. |
| Description | Country affiliation: United kingdom Author Affiliation: Penchala SD ( Department of Molecular & Clinical Pharmacology, University of Liverpool, Liverpool, UK.) |
| Abstract | A sensitive high-performance reverse phase liquid chromatography-positive ion electrospray tandem mass spectrometry method was developed and validated for the quantification of telaprevir and its inactive R-diastereomer (VRT-127394) in human plasma. The analytes and the internal standard (telaprevir-d11) were extracted from plasma by liquid-liquid extraction using tert-Butyl methyl ether (TBME). Chromatographic separation was achieved on a reversed-phase Accucore C18 column with a gradient programme consisting of water:ammonia (25%), 100:0.01 (v/v) (mobile phase A) and ACN:MeOH:ammonia (25%), 15:85:0.01 (v/v/v) (mobile phase B). The MS acquisition was performed with selective reaction monitoring mode using the respective [M+H](+) ions, m/z 680.59â322.42 for telaprevir and VRT-127394, and 691.15â110.13 for telaprevir-d11. The assay exhibited a linear dynamic range of 5-5000ng/mL for telaprevir and VRT-127394. Acceptable precision (%RSD<6.5%) and accuracy (94-108%) were obtained for concentrations over the range of the standard curve. A procedure was established to stabilise the plasma to prevent ex vivo interconversion of the isomers. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| Volume Number | 932 |
| e-ISSN | 1873376X |
| Journal | Journal of Chromatography B |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-08-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Antiviral Agents Blood Hepacivirus Enzymology Oligopeptides Protease Inhibitors Spectrometry, Mass, Electrospray Ionization Methods Chemistry Chromatography, Liquid Hepatitis C Drug Therapy Humans Limit Of Detection Stereoisomerism Tandem Mass Spectrometry Journal Article Research Support, Non-u.s. Gov't Validation Studies |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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