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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Huang, Liusheng Haagensen, Janus Verotta, Davide Lizak, Patricia Aweeka, Francesca Yang, Katherine |
| Description | Author Affiliation: Huang L ( Department of Clinical Pharmacy, University of California San Francisco School of Pharmacy, San Francisco, CA, USA. Electronic address: huangl@sfghsom.ucsf.edu.); Haagensen J ( Department of Civil and Environmental Engineering, Stanford University, Palo Alto, CA, USA.); Verotta D ( Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, School of Pharmacy, San Francisco, CA, USA.); Lizak P ( Department of Clinical Pharmacy, University of California San Francisco School of Pharmacy, San Francisco, CA, USA.); Aweeka F ( Department of Clinical Pharmacy, University of California San Francisco School of Pharmacy, San Francisco, CA, USA.); Yang K ( Department of Clinical Pharmacy, University of California San Francisco School of Pharmacy, San Francisco, CA, USA.) |
| Abstract | To support the development of a dynamic in vitro human pharmacokinetic/pharmacodynamic simulation model for biofilm-mediated infections and study stability of meropenem, an LC-MS/MS method for the determination of meropenem in Luria Bertani (LB) media was developed and validated in an API2000 LC-MS/MS system. A partial validation was also performed in M9 media. Sample aliquots of 100µL (or 25µL for M9 media) were mixed with the internal standard (IS) ceftazidime and filtered. The filtrate was directly injected onto a C8 column eluted with ammonium formate (10mM, pH 4) and acetonitrile (0.1% formic acid) in a gradient mode. ESI(+) and MRM with ion pair m/z 384â68 for meropenem and m/z 547â468 for the IS were used for quantification. The calibration curve concentration range was 50 to 25,000ng/mL. The recovery was over 98%. In LB media, significant signal suppression was observed throughout the time period of detection when compared with mobile phase solvents, but the matrix effect was compensated well with the IS. In M9 media, much less signal suppression was observed. The method is simple, fast, and reliable. Using the method, stability of meropenem in LB and M9 media were tested. No significant degradation was observed for at least 8h in both LB media (37°C) and M9 media (30°C), but more than 15% degradation was observed overnight (â¼20h). The method was transferred to an API5000 LC-MS/MS system using meropenem-d6 as the IS. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| e-ISSN | 1873376X |
| DOI | 10.1016/j.jchromb.2014.05.002 |
| Journal | Journal of Chromatography B |
| Volume Number | 961 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-06-15 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Discipline Analytical Chemistry Culture Media Chemistry Thienamycins Calibration Chromatography, Liquid Drug Stability Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry Comparative Study Research Support, N.i.h., Extramural Validation Studies |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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