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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Schiavone, Nicole M. Sarver, Scott A. Sun, Liangliang Wojcik, Roza Dovichi, Norman J. |
| Description | Country affiliation: United States Author Affiliation: Schiavone NM ( Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA.); Sarver SA ( Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA.); Sun L ( Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA.); Wojcik R ( Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA.); Dovichi NJ ( Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA. Electronic address: ndovichi@nd.edu.) |
| Abstract | While capillary zone electrophoresis (CZE) has been used to produce very rapid and efficient separations, coupling these high-speed separations with mass spectrometry (MS) has been challenging. Now, with much faster and sensitive mass spectrometers, it is possible to take full advantage of the CZE speed and reconstruct the fast migrating peaks. Here are three high-speed CZE-MS analyses via an electrokinetically pumped sheath-flow interface. The first separation demonstrates CZE-ESI-MS of an amino acid mixture with a 2-min separation, >50,000 theoretical plates, low micromolar concentration detection limits, and subfemtomole mass detection limits (LTQ XL mass spectrometer). The second separation with our recently improved third-generation CE-MS interface illustrates a 20 amino acid separation in â¼7min with an average over 200,000 plate counts, and results in almost-baseline resolution of structural isomers, leucine and isoleucine. The third separation is of a BSA digest with a reproducible CZE separation and mass spectrometry detection in 2min. CZE-MS/MS analysis of the BSA digest identified 31 peptides, produced 52% sequence coverage, and generated a peak capacity of â¼40 across the 1-min separation window (Q-Exactive mass spectrometer). |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| e-ISSN | 1873376X |
| DOI | 10.1016/j.jchromb.2015.04.001 |
| Journal | Journal of Chromatography B |
| Volume Number | 991 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-06-01 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Discipline Analytical Chemistry Amino Acids Electrophoresis, Capillary Serum Albumin, Bovine Chemistry Tandem Mass Spectrometry Isolation & Purification Animals Spectrometry, Mass, Electrospray Ionization Time Factors Research Support, N.i.h., Extramural |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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