| Author |
Abedini, Amin Chollet, Sébastien Angelis, Apostolis Borie, Nicolas Nuzillard, Jean-Marc Skaltsounis, Alexios-Leandros Reynaud, Romain Gangloff, Sophie C. Renault, Jean-Hugues Hubert, Jane |
| Description |
Country affiliation: France Author Affiliation: Abedini A ( Institut de Chimie Moléculaire de Reims, UMR CNRS 7312, SFR CAP'santé, UFR de Pharmacie, Université de Reims Champagne-Ardenne, Reims, France); Chollet S ( Institut de Chimie Moléculaire de Reims, UMR CNRS 7312, SFR CAP'santé, UFR de Pharmacie, Université de Reims Champagne-Ardenne, Reims, France); Angelis A ( Institut de Chimie Moléculaire de Reims, UMR CNRS 7312, SFR CAP'santé, UFR de Pharmacie, Université de Reims Champagne-Ardenne, Reims, France); Borie N ( Institut de Chimie Moléculaire de Reims, UMR CNRS 7312, SFR CAP'santé, UFR de Pharmacie, Université de Reims Champagne-Ardenne, Reims, France.); Nuzillard JM ( Institut de Chimie Moléculaire de Reims, UMR CNRS 7312, SFR CAP'santé, UFR de Pharmacie, Université de Reims Champagne-Ardenne, Reims, France.); Skaltsounis AL ( Division of Pharmacognosy and Natural Products Chemistry, School of Pharmacy, University of Athens, Panepistimioupolis, Zographou, Athens, Greece.); Reynaud R ( Soliance-Givaudan SAS, Route de Bazancourt, 51110 Pomacle, France.); Gangloff SC ( Biomatériaux et Inflammation en Site Osseux, EA 4691, SFR CAP'santé, UFR de Pharmacie, Université de Reims Champagne Ardenne, Reims, France.); Renault JH ( Institut de Chimie Moléculaire de Reims, UMR CNRS 7312, SFR CAP'santé, UFR de Pharmacie, Université de Reims Champagne-Ardenne, Reims, France.); Hubert J ( Institut de Chimie Moléculaire de Reims, UMR CNRS 7312, SFR CAP'santé, UFR de Pharmacie, Université de Reims Champagne-Ardenne, Reims, France. Electronic address: jane.hubert@univ-reims.fr.) |
| Abstract |
Barks from conifers and broadleaved trees constitute abundant wastes generated from wood harvesting and logging activities. Extracts of such residues obtained from Alnus trees have been reported as interesting resources with potent antibacterial activities. The present study aims to determine the antimicrobial activity of a crude methanol extract prepared from the bark of Alnus glutinosa against a panel of 22 bacteria and yeasts and to optimize a purification method enabling the high production of the most active substances. Fractionation of the crude extract was performed by Centrifugal Partition Chromatography (CPC) using a three-phase solvent system composed of n-heptane, methyl-ter-butyl ether, acetonitrile and water. The major known compounds contained in the fractions produced by CPC were chemically profiled by (13)C NMR dereplication, resulting in the unambiguous identification of oregonin, hirsutanonol, betulinic acid, and alusenone 1a. The antibacterial evaluation of the fractions by bioautography on Staphylococcus aureus revealed that oregonin, in addition to being the major metabolite of the crude extract (â¼32% w/w), was the most active with an antibacterial inhibitory effect comparable to antibiotics. The purification of oregonin was optimized at the laboratory-scale by CPC. A single injection of 3.7g of crude extract resulted in a recovery of 72% (850mg) of the available oregonin at purity higher than 94%. |
