NDLI: Local transplantation is an effective method for cell delivery in the osteogenesis imperfecta murine model.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Pauley, Penelope Matthews, Brya G. Wang, Liping Dyment, Nathaniel A. Matic, Igor Rowe, David W. Kalajzic, Ivo
Description	Country affiliation: United States Author Affiliation: Pauley P ( Department of Reconstructive Sciences, MC 3705, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT, 06032, USA.)
Abstract	PURPOSE: Osteogenesis imperfecta is a serious genetic disorder that results from improper type I collagen production. We aimed to evaluate whether bone marrow stromal cells (BMSC) delivered locally into femurs were able to engraft, differentiate into osteoblasts, and contribute to formation of normal bone matrix in the osteogenesis imperfect murine (oim) model. METHODS: Donor BMSCs from bone-specific reporter mice (Col2.3GFP) were expanded in vitro and transplanted into the femoral intramedullary cavity of oim mice. Engraftment was evaluated after four weeks. RESULTS: We detected differentiation of donor BMSCs into Col2.3GFP+ osteoblasts and osteocytes in cortical and trabecular bone of transplanted oim femurs. New bone formation was detected by deposition of dynamic label in the proximity to the Col2.3GFP+ osteoblasts, and new bone showed more organized collagen structure and expression of type I 2 collagen. Col2.3GFP cells were not found in the contralateral femur indicating that transplanted osteogenic cells did not disseminate by circulation. No osteogenic engraftment was observed following intravenous transplantation of BMSCs. BMSC cultures derived from transplanted femurs showed numerous Col2.3GFP+ colonies, indicating the presence of donor progenitor cells. Secondary transplantation of cells recovered from recipient femurs and expanded in vitro also showed Col2.3GFP+ osteoblasts and osteocytes confirming the persistence of donor stem/progenitor cells. CONCLUSION: We show that BMSCs delivered locally in oim femurs are able to engraft, differentiate into osteoblasts and osteocytes and maintain their progenitor potential in vivo. This suggests that local delivery is a promising approach for introduction of autologous MSC in which mutations have been corrected.
File Format	HTM / HTML
ISSN	03412695
e-ISSN	14325195
DOI	10.1007/s00264-013-2249-y
Journal	International Orthopaedics
Issue Number	9
Volume Number	38
Language	English
Publisher	Springer
Publisher Date	2014-09-01
Publisher Place	Germany
Access Restriction	Open
Subject Keyword	Discipline Orthopedics Disease Models, Animal Mesenchymal Stem Cell Transplantation Mesenchymal Stromal Cells Osteogenesis Imperfecta Therapy Animals Cell Differentiation Femur Pathology Surgery Mice Mice, Inbred C57bl Mice, Mutant Strains Osteoblasts Osteoclasts Osteogenesis Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Orthopedics and Sports Medicine Surgery

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Local Transplantation is an Effective Method for Cell Delivery in the Osteogenesis Imperfecta Murine Model

Total body irradiation is permissive for mesenchymal stem cell-mediated new bone formation following local transplantation.

Biologic characteristics and osteogenic differentiation of maxillary primordium mesenchymal cells.

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