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Identifying gene regulatory elements by genomic microarray mapping of DNaseI hypersensitive sites.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Follows, George A. Dhami, Pawan Göttgens, Berthold Bruce, Alexander W. Campbell, Peter J. Dillon, Shane C. Smith, Aileen M. Koch, Christoph Donaldson, Ian J. Scott, Mike A. Dunham, Ian Janes, Mary E. Vetrie, David Green, Anthony R.
Description	Country affiliation: United kingdom Author Affiliation: Follows GA ( Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, CB2 2XY, United Kingdom. gf246@cam.ac.uk)
Abstract	The identification of cis-regulatory elements is central to understanding gene transcription. Hypersensitivity of cis-regulatory elements to digestion with DNaseI remains the gold-standard approach to locating such elements. Traditional methods used to identify DNaseI hypersensitive sites are cumbersome and can only be applied to short stretches of DNA at defined locations. Here we report the development of a novel genomic array-based approach to DNaseI hypersensitive site mapping (ADHM) that permits precise, large-scale identification of such sites from as few as 5 million cells. Using ADHM we identified all previously recognized hematopoietic regulatory elements across 200 kb of the mouse T-cell acute lymphocytic leukemia-1 (Tal1) locus, and, in addition, identified two novel elements within the locus, which show transcriptional regulatory activity. We further validated the ADHM protocol by mapping the DNaseI hypersensitive sites across 250 kb of the human TAL1 locus in CD34+ primary stem/progenitor cells and K562 cells and by mapping the previously known DNaseI hypersensitive sites across 240 kb of the human alpha-globin locus in K562 cells. ADHM provides a powerful approach to identifying DNaseI hypersensitive sites across large genomic regions.
File Format	HTM / HTML
ISSN	10889051
e-ISSN	15495469
Journal	Genome Research
Issue Number	10
Volume Number	16
Language	English
Publisher	Cold Spring Harbor Laboratory Press
Publisher Date	2006-10-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Genetics Discipline Genomics Deoxyribonuclease I Metabolism Genomics Microarray Analysis Regulatory Elements, Transcriptional Genetics Restriction Mapping Algorithms Animals Basic Helix-loop-helix Transcription Factors Evaluation Studies As Topic Mice Proto-oncogene Proteins Ubiquitin-protein Ligases Comparative Study Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Genetics (clinical)

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Identifying gene regulatory elements by genomic microarray mapping of DNaseI hypersensitive sites.

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Identifying gene regulatory elements by genomic microarray mapping of DNaseI hypersensitive sites

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Identifying gene regulatory elements by genomic microarray mapping of DNaseI hypersensitive sites.