NDLI: Hypothetical protein Rv3423.1 of Mycobacterium tuberculosis is a histone acetyltransferase.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Jose, Leny Ramachandran, Ranjit Bhagavat, Raghu Gomez, Roshna Lawrence Chandran, Aneesh Raghunandanan, Sajith Omkumar, Ramakrishnapillai Vyomakesannair Chandra, Nagasuma Mundayoor, Sathish Kumar, Ramakrishnan Ajay
Description	Country affiliation: India Author Affiliation: Jose L ( Mycobacterium Research Group, Tropical Disease Biology Division, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, India.); Ramachandran R ( Mycobacterium Research Group, Tropical Disease Biology Division, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, India.); Bhagavat R ( Bioinformatics Centre, Indian Institute of Science, Bangalore, India.); Gomez RL ( Mycobacterium Research Group, Tropical Disease Biology Division, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, India.); Chandran A ( Mycobacterium Research Group, Tropical Disease Biology Division, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, India.); Raghunandanan S ( Mycobacterium Research Group, Tropical Disease Biology Division, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, India.); Omkumar RV ( Neurobiology Group, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, India.); Chandra N ( Bioinformatics Centre, Indian Institute of Science, Bangalore, India.); Mundayoor S ( Mycobacterium Research Group, Tropical Disease Biology Division, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, India.); Kumar RA ( Mycobacterium Research Group, Tropical Disease Biology Division, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, India.)
Abstract	We isolated an 8 kDa mycobacterial hypothetical protein, Rv3423.1, from the chromatin of human macrophages infected with Mycobacterium tuberculosis H37Rv. Bioinformatics predictions followed by in vitro biochemical assays with purified recombinant protein showed that Rv3423.1 is a novel histone acetyltransferase that acetylates histone H3 at the K9/K14 positions. Transient transfection of macrophages containing GFP-tagged histone H1 with RFP-tagged Rv3423.1 revealed that the protein co-localizes with the chromatin in the nucleus. Co-immunoprecipitation assays confirmed that the Rv3423.1-histone interaction is specific. Rv3423.1 protein was detected in the culture filtrate of virulent but not avirulent M. tuberculosis. Infection of macrophages with recombinant Mycobacterium smegmatis constitutively expressing Rv3423.1 resulted in a significant increase in the number of intracellular bacteria. However, the protein did not seem to offer any growth advantage to free-living recombinant M. smegmatis. It is highly likely that, by binding to the host chromatin, this histone acetyltransferase from M. tuberculosis may manipulate the expression of host genes involved in anti-inflammatory responses to evade clearance and to survive in the intracellular environment.
File Format	HTM / HTML
ISSN	1742464X
Issue Number	2
Volume Number	283
e-ISSN	17424658
Journal	FEBS Journal
Language	English
Publisher	Wiley (on behalf of the Federation of European Biochemical Societies)
Publisher Date	2016-01-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biochemistry Bacterial Proteins Genetics Metabolism Histone Acetyltransferases Mycobacterium Tuberculosis Enzymology Acetyl Coenzyme A Chemistry Chromatin Computer Simulation Gene Expression Regulation, Bacterial Histones Humans Macrophages Microbiology Mycobacterium Smegmatis Pathogenicity Nad Protein Conformation Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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