NDLI: Computerized sperm motility analysis in toxicity bioassays: a new approach to pore water quality assessment.

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Computerized sperm motility analysis in toxicity bioassays: a new approach to pore water quality assessment.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Fabbrocini, Adele Di Stasio, Michele D'Adamo, Raffaele
Spatial Coverage	Italy
Description	Country affiliation: Italy Author Affiliation: Fabbrocini A ( Consiglio Nazionale delle Ricerche-Istituto di Scienze Marine, UOS Lesina, via Pola 4, 71010 Lesina (FG), Italy. adele.fabbrocini@fg.ismar.cnr.it)
Abstract	The aim of this study was to test the sensitivity of computerized sperm motility analysis in the sea urchin Paracentrotus lividus as the endpoint in toxicity bioassays. The tested matrices were pore water samples collected in an agriculture-impacted Mediterranean lagoon, Lake Varano (Italy). Two standardized bioassays were also conducted as controls, the P. lividus spermiotoxicity test and the Vibrio fischeri (Microtox®) test. VCL (curvilinear velocity), VSL (straight line velocity), VAP (average path velocity), and the percentage of rapid spermatozoa recorded by the Sperm Class Analyzer® system showed high sensitivity and discrimination ability, to a degree comparable with the larval development endpoint of the spermiotoxicity test. The test evaluated in this study requires small volumes of matrices, involves minimal sample manipulation, and can easily be extended to many other bioindicator species. It may therefore be considered a promising 'quick response tool' following hazardous events that may adversely affect an aquatic ecosystem.
File Format	HTM / HTML
ISSN	01476513
Issue Number	7
Volume Number	73
e-ISSN	10902414
Journal	Ecotoxicology and Environmental Safety
Language	English
Publisher	Elsevier
Publisher Date	2010-10-01
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Environmental Sciences Biological Assay Methods Fresh Water Chemistry Sea Urchins Drug Effects Sperm Motility Toxicity Tests Water Pollutants, Chemical Analysis Aliivibrio Fischeri Analysis Of Variance Animals Italy Male Sensitivity And Specificity Comparative Study Evaluation Studies Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Pollution Health, Toxicology and Mutagenesis Public Health, Environmental and Occupational Health

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Computerized sperm motility analysis in toxicity bioassays: a new approach to pore water quality assessment.

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