NDLI: Cancellous bone properties and matrix content of TGF-beta2 and IGF-I in human tibia: a pilot study.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yeni, Yener N. Dong, X. Neil Zhang, Bingbing Gibson, Gary J. Fyhrie, David P.
Description	Country affiliation: United States Author Affiliation: Yeni YN ( Department of Orthopaedics and Rehabilitation, Section of Biomechanics, Bone and Joint Center, Henry Ford Hospital, 2799 West Grand Boulevard, E&R 2015, Detroit, MI 48202, USA. yeni@bjc.hfh.edu)
Abstract	Transforming and insulin-like growth factors are important in regulating bone mass. Thus, one would anticipate correlations between matrix concentrations of growth factors and functional properties of bone. We therefore investigated the relationships of (1) TGF-beta2 and (2) IGF-I matrix concentrations with the trabecular microstructure, stress distribution, and mechanical properties of tibial cancellous bone from six male human cadavers. Trabecular stress amplification (VMExp/sigma(app)) and variability (VMCOV) were calculated using microcomputed tomography (muCT)-based finite element simulations. Bone volume fraction (BV/TV), surface/volume ratio (BS/BV), trabecular thickness (Tb.Th), number (Tb.N) and separation (Tb.Sp), connectivity (Eu.N), and anisotropy (DA) were measured using 3-D morphometry. Bone stiffness and strength were measured by mechanical testing. Matrix concentrations of TGF-beta2 and IGF-I were measured by ELISA. We found higher matrix concentrations of TGF-beta2 were associated with higher Tb.Sp and VMExp/sigma(app) for pooled data and within subjects. Similarly, a higher matrix concentration of IGF-I was associated with lower stiffness, strength, BV/TV and Tb.Th and with higher BS/BV, Tb.Sp, VMExp/sigma(app) and VMCOV for pooled data and within subjects. IGF-I and Tb.N were negatively associated within subjects. It appears variations of the stress distribution in cancellous bone correlate with the variation of the concentrations of TGF-beta2 and IGF-I in bone matrix: increased local matrix concentrations of growth factors are associated with poor biomechanical and architectural properties of tibial cancellous bone.
File Format	HTM / HTML
ISSN	0009921X
e-ISSN	15281132
DOI	10.1007/s11999-009-0896-5
Journal	Clinical Orthopaedics and Related Researchtextregistered
Issue Number	12
Volume Number	467
Language	English
Publisher	Springer
Publisher Date	2009-12-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Orthopaedics Bone Matrix Chemistry Insulin-like Growth Factor I Tibia Transforming Growth Factor Beta2 Biomechanical Phenomena Cadaver Computer Simulation Elastic Modulus Enzyme-linked Immunosorbent Assay Finite Element Analysis Imaging, Three-dimensional Models, Anatomic Pilot Projects Stress, Mechanical X-ray Microtomography
Content Type	Text
Resource Type	Article
Subject	Orthopedics and Sports Medicine Surgery Sports Science

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