NDLI: Correlation of LINE-1 methylation levels in patient-matched buffy coat, serum, buccal cell, and bladder tumor tissue DNA samples.

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Correlation of LINE-1 methylation levels in patient-matched buffy coat, serum, buccal cell, and bladder tumor tissue DNA samples.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Van Bemmel, Dana Lenz, Petra Liao, Linda M. Baris, Dalsu Sternberg, Lawrence R. Warner, Andrew Johnson, Alison Jones, Michael Kida, Masatoshi Schwenn, Molly Schned, Alan R. Silverman, Debra T. Rothman, Nathaniel Moore, Lee E.
Description	Country affiliation: United States Author Affiliation: van Bemmel D ( Occupational and Environmental Epidemiology Branch, Epidemiology and Biostatistics Program, National Cancer Institute, NIH, DHHS, Bethesda, MD 20852, USA.)
Abstract	BACKGROUND: Evidence suggests that global methylation levels in blood cell DNA may be a biomarker for cancer risk. To date, most studies have used genomic DNA isolated from blood or urine as a surrogate marker of global DNA methylation levels in bladder tumor tissue. METHODS: A subset of 50 bladder cancer cases was selected from the New England Bladder Cancer Case-Control Study. Genomic DNA was isolated from buffy coat, buccal cells, serum, and formalin-fixed, paraffin-embedded tissue for each participant. DNA methylation at four CpG sites within the long interspersed nucleotide element (LINE-1) repetitive element was quantified using pyrosequencing and expressed as a mean methylation level across sites. RESULTS: Overall, the mean percent (%) LINE-1 5-methylcytosine (%5MeC) level was highest in serum (80.47% ± 1.44%) and lowest in bladder tumor DNA (61.36% ± 12.74%) and levels varied significantly across tissue types (P = 0.001). An inverse association between LINE-1 mean %5MeC and tumor stage (P = 0.001) and grade (P = 0.002) was observed. A moderate correlation between patient-matched serum and buffy coat DNA LINE-1 %5MeC levels was found (r = 0.32, P = 0.03) but levels were uncorrelated among other matched genomic DNA samples. CONCLUSIONS: The mean promoter LINE-1 %5MeC measurements were correlated between buffy coat and serum DNA samples. No correlation was observed between genomic DNA sources and tumor tissues; however a significant inverse association between tumor percent LINE-1 methylation and tumor stage/grade was found. IMPACT: LINE-1 methylation measured in case blood DNA did not reflect that observed in bladder tumor tissue but may represent other factors associated with carcinogenesis.
File Format	HTM / HTML
ISSN	10559965
e-ISSN	15387755
DOI	10.1158/1055-9965.EPI-11-1030
Journal	Cancer Epidemiology Biomarkers & Prevention
Issue Number	7
Volume Number	21
Language	English
Publisher	American Association for Cancer Research
Publisher Date	2012-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Cancer epidemiology Tumor Markers, Biological Genetics Blood Cells Metabolism Dna Methylation Long Interspersed Nucleotide Elements Mouth Mucosa Urinary Bladder Neoplasms Urinary Bladder Pathology Case-control Studies Neoplasm Grading Neoplasm Staging Polymerase Chain Reaction Prognosis Survival Rate Diagnosis Mortality Comparative Study Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Epidemiology Oncology

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