NDLI: Strain improvement of Streptomyces roseosporus for daptomycin production by rational screening of He-Ne laser and NTG induced mutants and kinetic modeling.

Please wait, while we are loading the content...

Strain improvement of Streptomyces roseosporus for daptomycin production by rational screening of He-Ne laser and NTG induced mutants and kinetic modeling.

Year: 2011 Volume: 163 Issue: 5

Year: 2011 Volume: 163 Issue: 4

Year: 2011 Volume: 163 Issue: 3

Year: 2011 Volume: 163 Issue: 2

Year: 2011 Volume: 163 Issue: 1

Year: 2010 Volume: 162

Year: 2010 Volume: 161

Year: 2010 Volume: 160

Year: 2009 Volume: 159

Year: 2009 Volume: 158

Year: 2009 Volume: 156

Year: 2009 Volume: 155

Year: 2009 Volume: 154

Year: 2009 Volume: 153

Year: 2008 Volume: 151

Year: 2008 Volume: 150

Year: 2008 Volume: 149

Year: 2008 Volume: 148

Year: 2008 Volume: 146

Year: 2008 Volume: 145

Year: 2008 Volume: 144

Year: 2007 Volume: 137-140

Year: 2006 Volume: 131

Year: 1982 Volume: 7

Year: 1981 Volume: 6

Strain improvement of Streptomyces roseosporus for daptomycin production by rational screening of He-Ne laser and NTG induced mutants and kinetic modeling.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yu, Guanghai Jia, Xiaoqiang Wen, Jianping Lu, Wenyu Wang, Guoying Caiyin, Qinggele Chen, Yunlin
Description	Author Affiliation: Yu G ( Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, People's Republic of China.)
Abstract	To improve the yield of daptomycin by Streptomyces roseosporus, a method of rational screening of He-Ne Laser and N-methyl-N-nitro-N-nitrosoguanidine (NTG)-induced mutants was employed in this work. Under the optimal mutagenesis conditions (NTG of 0.3 mg/L, 40 min; irradiation at 15 mW, 15 min), two steps of combined mutations were conducted. Screening of mutants was done according to the individual resistance to n-decanoic acid and daptomycin. A mutant strain LC-54-16, with the highest daptomycin production ability of 616 mg/L was obtained, which was over 5 times higher than the wild strain LC-52-6. The transcription levels of the main dpt genes in the mutant were approximately five times higher than those in the wild. The superiority of the mutant to the wild strain was further proved by the comparative studies on the kinetics of the mutant and the wild. The decrease of the inhibition of substrate and product was further confirmed, as well. It was concluded that the method of rational screening of He-Ne Laser and NTG-induced mutants could efficiently improve the daptomycin production ability of S. roseosporus.
File Format	HTM / HTML
ISSN	02732289
Issue Number	6
Volume Number	163
e-ISSN	15590291
Journal	Applied Biochemistry and Biotechnology
Language	English
Publisher	Springer
Publisher Date	2011-03-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biochemistry Discipline Biotechnology Biotechnology Methods Daptomycin Biosynthesis Genetic Testing Lasers, Gas Models, Biological Mutation Genetics Streptomyces Bioreactors Microbiology Confidence Intervals Chemistry Pharmacology Decanoic Acids Drug Resistance, Fungal Drug Effects Fermentation Gene Expression Regulation, Fungal Kinetics Methylnitronitrosoguanidine Microbial Viability Mutagenesis Reproducibility Of Results Reverse Transcriptase Polymerase Chain Reaction Time Factors Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Strain Improvement of Streptomyces roseosporus for Daptomycin Production by Rational Screening of He–Ne Laser and NTG Induced Mutants and Kinetic Modeling

Kinetic Analysis and Modeling of Daptomycin Batch Fermentation by Streptomyces roseosporus

Transcriptional analysis of the effect of exogenous decanoic acid stress on Streptomyces roseosporus

Kinetic analysis and modeling of daptomycin batch fermentation by Streptomyces roseosporus.

Enhancement of daptomycin production in Streptomyces roseosporus LC-51 by manipulation of cofactors concentration in the fermentation culture

Improvement of daptomycin production via increased resistance to decanoic acid in Streptomyces roseosporus.

Genome Shuffling of Streptomyces roseosporus for Improving Daptomycin Production

Metabolic Flux Analysis and Principal Nodes Identification for Daptomycin Production Improvement by Streptomyces roseosporus

Genome shuffling of Streptomyces roseosporus for improving daptomycin production.

Strain improvement of Streptomyces roseosporus for daptomycin production by rational screening of He-Ne laser and NTG induced mutants and kinetic modeling.

Similar Documents

Strain Improvement of Streptomyces roseosporus for Daptomycin Production by Rational Screening of He–Ne Laser and NTG Induced Mutants and Kinetic Modeling

Kinetic Analysis and Modeling of Daptomycin Batch Fermentation by Streptomyces roseosporus

Transcriptional analysis of the effect of exogenous decanoic acid stress on Streptomyces roseosporus

Kinetic analysis and modeling of daptomycin batch fermentation by Streptomyces roseosporus.

Enhancement of daptomycin production in Streptomyces roseosporus LC-51 by manipulation of cofactors concentration in the fermentation culture

Improvement of daptomycin production via increased resistance to decanoic acid in Streptomyces roseosporus.

Genome Shuffling of Streptomyces roseosporus for Improving Daptomycin Production

Metabolic Flux Analysis and Principal Nodes Identification for Daptomycin Production Improvement by Streptomyces roseosporus

Genome shuffling of Streptomyces roseosporus for improving daptomycin production.

Strain improvement of Streptomyces roseosporus for daptomycin production by rational screening of He-Ne laser and NTG induced mutants and kinetic modeling.