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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bonsegna, Stefania Bettini, Simona Pagano, Rosanna Zacheo, Antonella Vergaro, Viviana Giovinazzo, Giovanna Caminati, Gabriella Leporatti, Stefano Valli, Ludovico Santino, Angelo |
| Description | Country affiliation: Italy Author Affiliation: Bonsegna S ( Institute of Sciences of Food Production CNR Unit of Lecce, Italy.) |
| Abstract | Oil bodies (OBs) are specialised organelles ubiquitously detected in plant oil seeds, which serve as lipid storage compartments. OBs consist of a hydrophobic core of triacylglycerol (TAGs), surrounded by a monolayer of phospholipids (PLs) embedded with some specific proteins with a size ranging from 0.5 to 2 µm. In this work, we report an easy method to reconstitute OBs starting from their constituents and to encapsulate lipophilic molecules, i.e. the fluorescent fluorescein isothiocyanate (FITC) and carboxyfluorescein (CF), into reconstituted OBs. This methods allowed us to produce OBs 4- to 10-fold smaller (50-200 nm) than the native one and to obtain a good recovery (about 40%) of both the fluorescent compounds used in the present work. The properties of reconstituted OBs were investigated by a combination of Brewster angle microscopy, scanning force microscopy, ζ-potential techniques. OBs were stable and formed ordered monolayers when patterned on hydrophobic substrates whereas they showed a higher tendency to aggregate into larger, coalescing OBs when were deposited onto hydrophilic substrates or at the air/water interface. Furthermore, we verified the uptake of FITC-loaded OBs by the MCF-7 breast cancer cell line. Our results indicated that OBs could be envisaged as novel carriers to deliver hydrophobic bioactive compounds. |
| File Format | HTM / HTML |
| ISSN | 02732289 |
| Issue Number | 6 |
| Volume Number | 163 |
| e-ISSN | 15590291 |
| Journal | Applied Biochemistry and Biotechnology |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2011-03-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Discipline Biotechnology Inclusion Bodies Metabolism Lipids Chemistry Plant Oils Air Cell Line, Tumor Corylus Humans Intracellular Space Microscopy, Atomic Force Microscopy, Confocal Prunus Seeds Static Electricity Water Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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