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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Shangguan, Jiao-Jiao Fan, Li-Qiang Ju, Xin Zhu, Qing-Qing Wang, Fu-Jun Zhao, Jian Xu, Jian-He |
| Description | Author Affiliation: Shangguan JJ ( State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People's Republic of China.) |
| Abstract | A 1,080-bp cDNA (CGMCC 2873) encoding of a cold-active lipase of Aspergillus fumigatus (AFL67) was cloned and expressed in Escherichia coli for the first time. The new lipase, AFL67, was one-step purified by 8.30 folds through Ni-NTA affinity chromatography with a recovery of 86.8 %. The specific activity of purified AFL67 was 449 U mg(-1) on p-NP hexanoate. AFL67 preferentially hydrolyzed p-nitrophenyl esters of short- and medium-chain fatty acids, with p-nitrophenyl hexanoate the maximum. The optimum temperature and pH was 15 °C and 7.5, respectively. The purified AFL67 was stable at 10-25 °C for 30 min, and in the pH range of 6.0-9.0 for 16 h (at 4 °C). Its activity was increased by 47 and 50 %, in the presence of 10 % (v/v) ethanol and isopropanol, respectively. The new lipase AFL67 highly enantioselectively deacylated (S)- -acetoxyphenylacetic acid (APA) and o-Cl-APA, m-Cl-APA, and p-Cl-APA to (S)-mandelic acid and its derivates. These features render this cold-active novel lipase AFL67 attractive for biotechnological applications in the field of enantioselective synthesis of chiral mandelic acids, o-acylated mandelic acids, and their derivates and detergent additives. |
| File Format | HTM / HTML |
| ISSN | 02732289 |
| Issue Number | 7 |
| Volume Number | 168 |
| e-ISSN | 15590291 |
| Journal | Applied Biochemistry and Biotechnology |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2012-12-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Discipline Biotechnology Aspergillus Fumigatus Enzymology Lipase Genetics Metabolism Acetates Chemistry Cloning, Molecular Enzyme Stability Drug Effects Escherichia Coli Gene Expression Hydrogen-ion Concentration Hydrolysis Isolation & Purification Mandelic Acids Metals Pharmacology Plasmids Protease Inhibitors Recombinant Proteins Solvents Stereoisomerism Substrate Specificity Temperature Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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