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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Armada, M. Pilar García Jiménez, Almudena Losada, José Alonso, Beatriz Casado, Carmen M. |
| Description | Country affiliation: Spain Author Affiliation: Armada MP ( Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, Madrid, Spain. pilar.garcia.armada@upm.es) |
| Abstract | In this work, the bioelectrocatalytical properties and kinetic characteristics of new oxidase amperometric biosensors based on two different ferrocene functionalized carbosilane polymers, polydiallylmethylsilane (PDAMS) and polymethyldiundecenylsilane (PMDUS) are described. In the development of these biodevices, glucose oxidase has been used as example of oxidase enzyme, and two different immobilization procedures have been studied. The polymer-modified electrodes act as efficient transducers for glucose sensing in anodic and cathodic aerobic conditions and also in anodic anaerobic conditions, and this fact turns them into useful devices for a wide field of applications. PMDUS has shown to be the bioelectrocatalyst with best kinetic and analytical properties in aerobic media while PDAMS was better in anaerobic conditions. The best aerobic biosensor developed displayed a strictly linear range from 0 to 3.0 mM, a detection limit of 7.8 µM and a response time less than 2 s in an ascorbate interference free work potential interval. The apparent Michaelis-Menten constant was calculated to be 1.36 mM according to the Lineweaver-Burk equation. |
| File Format | HTM / HTML |
| ISSN | 02732289 |
| Issue Number | 7 |
| Volume Number | 168 |
| e-ISSN | 15590291 |
| Journal | Applied Biochemistry and Biotechnology |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2012-12-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Discipline Biotechnology Biocatalysis Biosensing Techniques Instrumentation Ferrous Compounds Chemistry Glucose Oxidase Metabolism Polymers Silanes Aerobiosis Anaerobiosis Animals Aspergillus Niger Enzymology Cattle Electrochemistry Electrodes Enzymes, Immobilized Glucose Analysis Kinetics Models, Molecular Platinum Protein Conformation Reproducibility Of Results Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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