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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Fang, Jianzheng Wang, Hainan Xi, Wei Cheng, Gong Wang, Shangqian Su, Shifeng Zhang, Shengli Deng, Yunfei Song, Zhen Xu, Aiming Liu, Bianjiang Cao, Jingyi Wang, Zengjun |
| Description | Country affiliation: China Author Affiliation: Fang J ( State Key Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, China.) |
| Abstract | Nuclear auto-antigenic sperm protein (NASP), initially described as a highly auto-immunogenic testis and sperm-specific protein, is a histone chaperone that is proved to present in all dividing cells. NASP has two splice variants: testicular NASP (tNASP) and somatic form of NASP (sNASP). Only cancer, germ, transformed, and embryonic cells have a high level of expression of the tNASP. Up to now, little has been known about tNASP in renal cell carcinoma (RCC). In the present study, the molecular mechanism of tNASP in RCC was explored. The expression level of tNASP in 16 paired human RCC specimens was determined. Downregulation of tNASP by small interfering RNA (siRNA) was transfected in RCC cell lines. The effect of downregulation of tNASP by siRNA on cell colony formation and proliferation was examined by colony formation assay and CCK-8 assay, cell cycle was analyzed by flow cytometry, and the expression of cyclin D1 and P21 were detected by Western blotting. ERK/MAPK signaling was also analyzed. tNASP has a relative high expression level in human RCC tissues. Via upregulation of P21 and downregulation of cyclinD1, silence of tNASP can inhibit cell proliferation, which induces cell cycle arrest. Furthermore, ERK signaling pathway is confirmed to mediate the regulation of cell cycle-related proteins caused by silence of tNASP. Our research demonstrates that knockdown of tNASP effectively inhibits the proliferation and causes G1 phase arrest through ERK/MAPK signal pathway. |
| File Format | HTM / HTML |
| ISSN | 10104283 |
| Issue Number | 7 |
| Volume Number | 36 |
| e-ISSN | 14230380 |
| Journal | Tumor Biology |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2015-07-01 |
| Publisher Place | Netherlands |
| Access Restriction | Subscribed |
| Subject Keyword | Discipline Medicine__semicolon__oncology Autoantigens Biosynthesis Carcinoma, Renal Cell Genetics Cell Proliferation Nuclear Proteins Pathology Cell Cycle Checkpoints Cell Cycle Proteins Cell Line, Tumor Cyclin D1 G1 Phase Gene Expression Regulation, Neoplastic Humans Map Kinase Signaling System Rna, Small Interfering P21-activated Kinases Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Cancer Research |
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