NDLI: TAB3 overexpression promotes cell proliferation in non-small cell lung cancer and mediates chemoresistance to CDDP in A549 cells via the NF-κB pathway.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chen, Jie Gu, Jun Feng, Jian Liu, Yifei Xue, Qun Ni, Tingting Wang, Zhiwen Jia, Liangliang Mao, Guoxin Ji, Lili
Description	Country affiliation: China Author Affiliation: Chen J ( Jiangsu Province Key Laboratory for Inflammation and Molecular Target, Medical College, Nantong University, Nantong, 226001, Jiangsu, China.); Gu J ( Department of Oncology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.); Feng J ( Department of Respiratory, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.); Liu Y ( Department of Oncology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.); Xue Q ( Department of Pathology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.); Ni T ( Department of Thoracic Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.); Wang Z ( Department of Oncology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.); Jia L ( Department of Thoracic Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.); Mao G ( Department of Respiratory Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210000, Jiangsu, China.); Ji L ( Department of Oncology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China. maoguoxinnt@126.com.)
Abstract	Transforming growth factor-activated kinase 1 (TAK1)-binding protein 3 (TAB3) is essential for the activation of the nuclear factor kappa B (NF-κB) pathway and has important roles in cell survival. However, the contribution of TAB3 to non-small cell lung cancer (NSCLC) remains elusive. In the present study, Western blotting and immunohistochemistry assays demonstrated that TAB3 expression was frequently increased in NSCLC tissues and cells. In addition, chi-square test and Kaplan-Meier analysis revealed that upregulation of TAB3 expression correlated with a more invasive tumor phenotype and poor prognosis. In addition, a series of experiments, including serum starvation-refeeding experiment and TAB3-siRNA transfection assay, showed that TAB3 expression promoted NSCLC cell proliferation. Furthermore, the effect of TAB3 expression on the sensitivity to cis-diamminedichloroplatinum (CDDP) and possible signaling transduction pathways was investigated. When the expression of TAB3 was inhibited by siRNA transfection, the sensitivity to CDDP was enhanced. Moreover, it showed that downregulation of TAB3 enhanced CDDP-induced A549 cell apoptosis through the inhibition of the NF-κB pathway. These results suggest that TAB3 plays a critical role in NSCLC progression and chemoresistance and that TAB3 depletion may be a promising approach to lung cancer therapy.
File Format	HTM / HTML
ISSN	10104283
Issue Number	3
Journal	Tumor Biology
Volume Number	37
e-ISSN	14230380
Language	English
Publisher	Springer
Publisher Date	2016-03-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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