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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ohshima, M. Itoh, H. Matsuoka, M. Murakami, T. Ohashi, Y. |
| Description | Country affiliation: Japan Author Affiliation: Ohshima M ( National Institute of Agrobiological Resources, Tsukuba Science City, Ibaraki, Japan.) |
| Abstract | The cis-acting elements for regulating gene expression of the tobacco pathogenesis-related 1a protein gene were analyzed in transgenic plants. The 5'-flanking 2.4-kilobase fragment from the pathogenesis-related 1a protein gene was joined to the bacterial beta-glucuronidase gene and introduced into tobacco cells by Agrobacterium-mediated gene transfer. Promoter activity was monitored by quantitative and histochemical assay of beta-glucuronidase activity in leaves of regenerated transgenic plants. The level of beta-glucuronidase activity was clearly increased by treatment with salicylic acid, by cutting stress, and by local lesion formation caused by tobacco mosaic virus infection. Cytochemical studies of the induced beta-glucuronidase activity revealed tissue-specific and developmentally regulated expression of the pathogenesis-related 1a gene after stress or chemical treatment and after pathogen attack. To identify the cis-acting element more precisely, a series of 5'-deleted chimeric genes was constructed and transformed into tobacco plants. Transgenic plants with a 0.3-kilobase fragment of the 5'-flanking region of the pathogenesis-related 1a gene had the same qualitative response as those with the 2.4-kilobase fragment upon treatment with salicylic acid or infection with TMV. Thus, the 0.3-kilobase DNA sequence fragment was sufficient to allow the regulated expression of the pathogenesis-related 1a gene. |
| File Format | HTM / HTML |
| ISSN | 10404651 |
| e-ISSN | 1531298X |
| Journal | THE PLANT CELL ONLINE |
| Issue Number | 2 |
| Volume Number | 2 |
| Language | English |
| Publisher | American Society of Plant Biologists |
| Publisher Date | 1990-02-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Botany Plant Proteins Genetics Plants, Genetically Modified Plants, Toxic Salicylates Pharmacology Tobacco Chromosome Deletion Enzyme Induction Drug Effects Gene Expression Regulation Genetic Vectors Glucuronidase Biosynthesis Isolation & Purification Histocytochemistry Kanamycin Resistance Molecular Sequence Data Growth & Development Regulatory Sequences, Nucleic Acid Salicylic Acid |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Plant Science |
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