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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bustos, M. M. Guiltinan, M. J. Jordano, J. Begum, D. Kalkan, F. A. Hall, T. C. |
| Description | Author Affiliation: Bustos MM ( Department of Biology, Texas A&M University, College Station 77843-3258.) |
| Abstract | A 0.8-kilobase fragment from the 5'-flanking region of a French bean beta-phaseolin gene yielded strong, temporally regulated, and embryo-specific expression of beta-glucuronidase (GUS) in transgenic tobacco plants, paralleling that found for the seed protein phaseolin [Sengupta-Gopalan, C., Reichert, N.A., Barker, R.F., Hall. T.C., and Kemp, J.D. (1985) Proc. Natl. Acad. Sci. USA 82, 3320-3324]. Gel retardation and footprinting assays using nuclear extracts from immature bean cotyledons revealed strong binding of nuclear proteins to an upstream region (-628 to -682) that contains two inverted A/T-rich motifs. Fusion of a 103-base pair fragment or a 55-base pair synthetic oligonucleotide containing these motifs to a minimal 35S promoter/GUS cassette yielded strong GUS expression in several tissues. A different pattern of GUS expression was obtained in immature embryos and germinating seedlings from the nominally constitutive, full-length, 35S promoter. Whereas GUS expression under the control of the 0.8-kilobase beta-phaseolin regulatory region is limited to immature embryos, expression from constructs containing the A/T-rich motifs is strongest in roots. These data, combined with S1 mapping, provide direct evidence that a plant upstream A/T-rich sequence that binds nuclear proteins can activate transcription in vivo. They also indicate that additional regulatory elements in the beta-phaseolin 5'-flanking region are required for embryo-specific gene expression. |
| File Format | HTM / HTML |
| ISSN | 10404651 |
| e-ISSN | 1531298X |
| Journal | THE PLANT CELL ONLINE |
| Issue Number | 9 |
| Volume Number | 1 |
| Language | English |
| Publisher | American Society of Plant Biologists |
| Publisher Date | 1989-09-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Botany Fabaceae Genetics Gene Expression Regulation, Enzymologic Glucuronidase Plant Proteins Plants, Medicinal Plants, Toxic Regulatory Sequences, Nucleic Acid Tobacco Base Composition Binding, Competitive Metabolism Kinetics Molecular Sequence Data Nuclear Proteins Organ Specificity Plants, Genetically Modified Restriction Mapping Transcription, Genetic Research Support, U.s. Gov't, Non-p.h.s. |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Plant Science |
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