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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhang, Haiyuan Lee, Moo-Yeal Hogg, Michael G. Dordick, Jonathan S. Sharfstein, Susan T. |
| Description | Country affiliation: United States Author Affiliation: Zhang H ( Department of Chemical and Biological Engineering and Center for Biotechnology and Interdisciplinary Science, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.) |
| Abstract | A method for high-throughput retroviral transfection of genes and interfering RNA into 3D cell-culture microarrays is described. 3D cultures more closely mimic the in vivo cellular milieu, thus providing cellular responses to genetic manipulation more similar to the in vivo situation than 2D cultures. This technique is applied to transfect several 'toxic' short-hairpin RNAs (shRNAs) into 3D cell cultures. It is demonstrated that the toxicity is similar to that obtained by conventional (non-high-throughput) retroviral transfection of cells grown in similar 3D culture microarrays. |
| File Format | HTM / HTML |
| ISSN | 16136810 |
| Issue Number | 13 |
| Volume Number | 8 |
| e-ISSN | 16136829 |
| Journal | Small |
| Language | English |
| Publisher | Wiley-VCH |
| Publisher Date | 2012-07-09 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Nanotechnology Transfection Methods Animals Cell Culture Techniques Cell Line Mice Nih 3t3 Cells Rna Interference Journal Article Research Support, U.s. Gov't, Non-p.h.s. |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Nanoscience and Nanotechnology Medicine Biomaterials Engineering Biotechnology |
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